Products > Crystallization Screens > Wizard Screen 1 • Wizard Screen 2 > Wizard Screen 1 • Wizard Screen 2 • Wizard Screen HT
Wizard Screen 1 • Wizard Screen 2 • Wizard Screen HT
Applications
- Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules
- Sparse matrix additive screen
Features
- Sparse matrix formula efficiently samples salts, polymers, volatile organics, non-volatile organics, & pH
- Tube or Deep Well block format
Description
Wizard Screen 1 and Wizard Screen 2 are a sparse matrix of trial crystallization reagent conditions developed by Steve L. Sarfaty and Wim G. J. Hol. The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).
Wizard Screen 1 is a complete reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules using a large range of precipitants, buffers, and salts, covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5. Wizard Screen 1 is a straightforward, effective, and practical kit for determining preliminary crystallization conditions. Wizard Screen 1 is also effective in determining the solubility of a macromolecule in a wide range of precipitants and pH.
Wizard Screen 2 is an extension of the reagents used in Wizard Screen 1, and is a complete reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules using a large range of precipitants, buffers, and salts, covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5. Wizard Screen 2 is a straightforward, effective, and practical kit for determining preliminary crystallization conditions. Wizard Screen 2 is also effective in determining the solubility of a macromolecule in a wide range of precipitants and pH.
Wizard Screen HT is comprised of Wizard Screen 1 and Wizard Screen 2 supplied in a sterile, polypropylene 96 Deep Well block, each reservoir containing 1 ml of sterile filtered reagent. The block is heat sealed using a special polypropylene backed film. Each Wizard Screen HT kit is supplied with an adhesive sealing film which can be used to seal the block after removing the heat seal. Additional adhesive sealing films can be obtained from Hampton Research or laboratory supply companies which offer high throughput plates and seals.
Support Material(s)
HR2-077 Wizard Screen 1 Documents HR2-077 Wizard Screen 1 SDS HR2-105 Wizard Screen 2 Documents HR2-105 Wizard Screen 2 SDS HR2-463 Wizard Screen HT Documents HR2-463 Wizard Screen HT SDS
Wizard Screen Formulation & Scoring Spreadsheet
Related Item(S)
References
1. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.
2. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.
3. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.