Products > Optimize Reagents > Optimize - Buffers > Tris Buffer
Tris Buffer
Applications
- Crystallization grade Tris for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Tris
Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 - 100.1%
pKa = 8.1 at 25°C
Useful pH range of Tris: 7.0 – 9.0
Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 - 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 - 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 - 1.35058 at 20°C
HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 - 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 - 1.35369 at 20°C
Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As ≤ 5 ppm max
Ca ≤ 5 ppm max
Cu ≤ 5 ppm max
Fe ≤ 5 ppm max
Pb ≤ 5 ppm max
Mg ≤ 5 ppm max
Trizma is a registered trademark of Sigma-Aldrich Co.
Support Material(s)
HR2-589 Tris SDSCertificate Of Analysis
Related Item(S)
References
1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.
