Products > Optimize Reagents > Optimize - Buffers > SPG Buffer
SPG Buffer
Applications
- Crystallization grade SPG buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
SPG is a novel, broad range buffer system designed to decouple the pH from buffer in crystallization and other experiments.
1.0 M Succinic acid / Sodium phosphate monobasic monohydrate / Glycine (SPG) is a buffer system useful across pH 4.0 - 10.0
The SPG buffer system is composed of 0.125 M Succinic acid, 0.438 M Sodium phosphate monobasic monohydrate, 0.437 M Glycine titrated to two stock pH‘s. 1.0 M SPG pH 4.0 is titrated using sodium hydroxide and 1.0 M SPG pH 10.0 is titrated using sodium hydroxide.
Using SPG, one can use a single buffer system to screen pH 4.0 to 10.0 by varying the ratio of SPG pH 4.0 to SPG pH 10.0. Each kit includes a 100 mL bottle of 1.0 M SPG pH 4.0, 100 mL bottle of 1.0 M SPG pH 10.0, and a user guide with a dilution table.
- 1.0 M Succinic acid / Sodium phosphate monobasic monohydrate / Glycine pH 4.0 (0.125 M Succinic acid / 0.438 M Sodium phosphate monobasic monohydrate / 0.437 M Glycine, titrated with sodium hydroxide to pH 4.0 at 25° Celsius.)
- 1.0 M Succinic acid / Sodium phosphate monobasic monohydrate / Glycine pH 10.0 (0.125 M Succinic acid / 0.438 M Sodium phosphate monobasic monohydrate / 0.437 M Glycine, titrated with Sodium hydroxide to pH 10.0 at 25° Celsius.)
Support Material(s)
HR2-493 1.0 M SPG SDSCertificate Of Analysis
Related Item(S)
References
1. Newman J. Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):610-2. doi: 10.1107/S0907444903029640. Epub 2004 Feb 25. PMID: 14993709.
2. Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL, Stuart DI, Perrakis A. Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31. doi: 10.1107/S0907444905024984. Epub 2005 Sep 28. PMID: 16204897.