Quik Screen

Applications

  • Primary or secondary, Sodium potassium phosphate versus pH grid crystallization screen for biological macromolecules

Features

  • A systematic grid screen varying Sodium potassium phosphate concentration versus pH
  • Samples pH 5 to 8.2
  • Samples four concentrations of Sodium potassium phosphate
  • Precise pH versus Sodium potassium phosphate screen
  • Combine reagents between rows or columns to create expanded grid screens

Description

Quik Screen is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Sodium potassium phosphate between pH 5.0 and 8.2.

Quik Screen evaluates Sodium potassium phosphate at four concentrations (0.8, 1.0, 1.4, 1.8 M) versus six pH levels (5.0, 5.6, 6.3, 6.9, 7.5, 8.2).

Quik Screen consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts. Individual reagents are available through the Hampton Research Custom Shop.



CAT NO

HR2-221

NAME

Quik Screen

DESCRIPTION

10 ml, tube format

PRICE

$124.00

Support Material(s)

Related Item(S)

References

1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.

2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.

4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

5. Crystallization and preliminary X-ray diffraction analysis of the glucuronoyl esterase catalytic domain from Hypocrea jecorina. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Apr 1; 64(Pt 4): 255-257.

6. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.