Products > Optimize Reagents > Optimize - Buffers > PCB Buffer
PCB Buffer
Applications
- Crystallization grade PCB buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
PCB is a novel, broad range buffer system designed to decouple the pH from buffer in crystallization and other experiments.
1.0 M Sodium propionate / Sodium cacodylate trihydrate / BIS-TRIS propane (PCB) is a buffer system useful across pH 4.0 - 9.5.
The PCB buffer system is composed of 0.4 M Sodium propionate / 0.2 M Sodium cacodylate trihydrate / 0.4 M BIS-TRIS propane titrated to two stock pH‘s. 1.0 M PCB pH 4.0 is titrated using hydrochloric acid and 1.0 M PCB pH 9.5 is titrated using sodium hydroxide.
Using PCB, one can use a single buffer system to screen pH 4.0 to 9.5 by varying the ratio of PCB pH 4.0 to PCB pH 10.0. Each kit includes a 100 mL bottle of 1.0 M PCB pH 4.0, 100 mL bottle of 1.0 M PCB pH 9.5, and a user guide with a dilution table.
- 1.0 Sodium propionate / Sodium cacodylate trihydrate / BIS-TRIS propane pH 4.0 (0.4 M Sodium propionate / 0.2 M Sodium cacodylate trihydrate / 0.4 M BIS-TRIS propane) is titrated with Hydrochloric acid to pH 4.0 at 25° Celsius.
- 1.0 Sodium propionate / Sodium cacodylate trihydrate / BIS-TRIS propane pH 9.5 (0.4 M Sodium propionate / 0.2 M Sodium cacodylate trihydrate / 0.4 M BIS-TRIS propane) titrated with Sodium hydroxide to pH 9.5 at 25° Celsius.
Support Material(s)
HR2-497 1.0 M PCB SDSCertificate Of Analysis
Related Item(S)
References
1. Newman J. Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):610-2. doi: 10.1107/S0907444903029640. Epub 2004 Feb 25. PMID: 14993709.
2. Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL, Stuart DI, Perrakis A. Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31. doi: 10.1107/S0907444905024984. Epub 2005 Sep 28. PMID: 16204897.