Products > Crystallization Screens > PACT > PACT • PACT HT
PACT • PACT HT
Applications
- Crystallization screen for proteins, peptides, nucleic acids and water soluble small molecules.
Features
- Systematic screen of pH, Anions, Cations & Polyethylene glycol
- 96 unique reagent formulations
- PEG vs pH; 24 wells
- PEG vs Cation; 24 wells
- PEG vs Anion 48 wells
- Tube or Deep Well block format
Description
PACT is a systematic crystallization screen evaluating pH, Anion, Cation, and Polyethylene glycol in 96 unique formulations.
Three screens in one. A 24 condition PEG/pH screen, a 24 condition PEG/cation screen and a 48 condition PEG/anion screen. The PEG/pH screen uses a multi-component buffer to cover pH 4 to 9 versus PEG, decoupling pH change and buffer. The PEG/cation screen evaluates six cations, with chloride counter-ions versus pH 5 to 8 in the presence of PEG. The PEG/anion screen evaluates twelve anions with sodium or potassium counter-ions versus pH 6.5 to 8.5 in the presence of PEG.
Support Material(s)
HR2-147 PACT Documents HR2-147 PACT SDS HR2-430 PACT HT Documents HR2-430 PACT HT SDS
PACT & PACT HT Formulations & Scoring
Related Item(S)
References
1. Newman et al (2005). Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. (2005). D61, 1426–1431.
2. Newman 2004. Novel buffer systems for macromolecular crystallization. Acta Cryst. (2004). D60, 610±612.