Products > Optimize Reagents > Optimize - Buffers > MES monohydrate Buffer

MES monohydrate Buffer

Applications

  • Crystallization grade MES monohydrate for formulating screens or for optimization

Features

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

MES monohydrate

Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate
C6H13NO4S • H2O
Mr 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 6350956
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity ≥ 99.0%
pKa 6.1 at 25°C

Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.

Useful pH range of MES 5.2 - 7.1

Titrate HR2-587 to the desired pH using NaOH
HR2-587 Measured pH Range: 3.2 - 3.4 at 25°C
HR2-587 Measured Conductivity Range: 166.4 - 2440 µS/cm at 25°C
HR2-587 Measured Refractive Index Range: 1.34655 - 1.34731 at 20°C

HR2-787 has been titrated to pH 6.5 using NaOH
HR2-787 Measured Conductivity Range: 19.2 - 25.6 mS/cm at 25°C
HR2-787 Measured Refractive Index Range: 1.36113 - 1.36251 at 20°C

HR2-943-07 - MES monohydrate pH 5.8 - 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 2 ppm max
As   ≤ 5 ppm max
Cl    ≤0.005%
Cu   ≤ 5 ppm max
Fe   ≤ 5 ppm max
Pb   ≤ 5 ppm max
SO4 ≤ 0.005%



CAT NO

HR2-587

NAME

0.5 M MES monohydrate

DESCRIPTION

100 mL

PRICE

$120.00

CAT NO

HR2-787

NAME

1.0 M MES monohydrate pH 6.5

DESCRIPTION

100 mL

PRICE

$120.00

Support Material(s)

Certificate Of Analysis

Related Item(S)

References

1. Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. Biochemistry 33, 1555- 1564, 1994.