Products > Custom Shop Crystallization Reagents > StockOptions PIPES > Individual StockOptions PIPES Reagents
Individual StockOptions PIPES Reagents
Applications
- Individual StockOptions PIPES reagents for screening, optimization, and production
Features
- 185 mL volume
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-257-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-257-01
PIPES titrated to indicated pH using NaOH
PIPES
Synonyms: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
C8H18N2O6S2
Mr 302.37
CAS Number [5625-37-6]
EC Number 227-057-6
Beilstein Registry 817713
Purity ≥ 99%
pKa 6.8
Useful pH range of PIPES buffer 6.1-7.5
Support Material(s)
HR2-257-** Individual StockOptions PIPES Reagents SDSRelated Item(S)
References
1. The expression, purification and crystallization of the epsilon subunit of the F1 portion of the ATPase of Escherichia coli. Codd, R; Cox, GB; Guss, JM; Solomon, RG; Webb, D. J Mol Biol 228, 306- 309, 1992.
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
3. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens
