Products > Optimize Reagents > Optimize - Buffers > Imidazole Buffer
Imidazole Buffer
Applications
- Crystallization grade Imidazole for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Imidazole
Synonyms: 1,3-Diaza-2,4-cyclopentadiene or Glyoxaline
C3H4N2
Mr 68.08
CAS Number [288-32-4]
EC Number 206-019-2
Beilstein Registry Number 103853
Merck 14,4912
RTECS NI3325000
MDL Number MFCD00005183
Purity ≥ 99.5%
UN Number 2923 8/PG 3
pKa = 6.95
The useful pH range of an Imidazole can be 6.2 - 7.8
HR2-819 is titrated to pH 7.0 using HCl
HR2-819 Measured Conductivity Range: 45.4 - 50.4 mS/cm at 25°C
HR2-819 Measured Refractive Index Range: 1.34836 - 1.34904 at 20°C
Titrate HR2-573 to the desired pH using HCl
HR2-573 Measured pH Range: 10.5 - 10.7 at 25°C
HR2-573 Measured Conductivity Range: 71.1 - 101.5 µS/cm at 25°C
HR2-573 Measured Refractive Index Range: 1.34420 - 1.34440 at 20°C
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Zn ≤0.0005%
Support Material(s)
HR2-573 1.0 M Imidazole SDSCertificate Of Analysis
Related Item(S)
References
1. Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions. X. Qiu and C. A. Janson. Acta Cryst. (2004). D60, 1545-1554 [doi:10.1107/S0907444904015422].
2. Protein crystallization by surface entropy reduction: optimization of the SER strategy. D. R. Cooper, T. Boczek, K. Grelewska, M. Pinkowska, M. Sikorska, M. Zawadzki and Z. Derewenda. Acta Cryst. (2007). D63, 636-645 [doi:10.1107/S0907444907010931].