Heavy Atom Screens

Applications

  • Heavy atoms for multiple isomorphous replacement

Features

  • Convenient sets of popular heavy atoms
  • Each kit include a heavy atom tutorial and formulation guide

Description

A Heavy Atom Screen kit provides 50 mg of each heavy atom in an o-ring screw cap micro tube. This convenient format provides sufficient material for the preparation of numerous (15 x 100 mM or 1,500 x 1 mM) small volume (0.1 ml) fresh stock solutions of heavy atoms. The quantity is sufficient for screening and derivatization but avoids the problem of storing large quantities of of heavy atoms.



CAT NO

HR2-442

NAME

Heavy Atom Screen Pt

DESCRIPTION

50 mg, tube format

PRICE

$527.00

CAT NO

HR2-444

NAME

Heavy Atom Screen Au

DESCRIPTION

50 mg, tube format

PRICE

$362.00

CAT NO

HR2-446

NAME

Heavy Atom Screen Hg

DESCRIPTION

50 mg, tube format

PRICE

$402.00

CAT NO

HR2-448

NAME

Heavy Atom Screen M1

DESCRIPTION

50 mg, tube format

PRICE

$440.00

CAT NO

HR2-450

NAME

Heavy Atom Screen M2

DESCRIPTION

50 mg, tube format

PRICE

$415.00

Support Material(s)

Related Item(S)

References

1. Towards a rational approach for heavy-atom derivative screening in protein crystallography. Johnson Agniswamy, M. Gordon Joyce, Carl H. Hammer, and Peter D. Sun. Acta Cryst. (2008) D64, 354-367.

2. Heavy-atom derivatization. Elspeth Garman and James W. Murray, Acta Cryst. (2003). D59, 1903-1913.

3. Screening for phasing atoms in protein crystallography. Titus J Boggon and Lawrence Shapiro. Structure 2000, Vol 8 No 7, 143-149.

4. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part II: phasing of new structures. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1099-103. Epub 2002 Jun 20. Sun PD, Radaev S.

5. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1092-8. Epub 2002 Jun 20. Sun PD, Radaev S, Kattah M.

6. Preparation of isomorphous heavy-atom derivatives. Methods Enzymol. 1985;114:147-56. Petsko GA. PMID: 4079763

7. An overview of heavy-atom derivatization of protein crystals. A. C. W. Pike, E. F. Garman, T. Krojer, F. von Delft & E. P. Carpenter (2016). Acta Cryst. D72, 303-318.

8. Fluorescence Detection of Heavy Atom Labeling (FD-HAL): a rapid method for identifying covalently modified cysteine residues by phasing atoms.Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J. J Struct Biol. 2010 Jul;171(1):82-7.

9. A rapid and rational approach to generating isomorphous heavy-atom phasing derivatives. Lu J, Sun PD. FEBS J. 2014 Sep;281(18):4021-8.

10. A rational approach to heavy-atom derivative screening. Joyce MG, Radaev S, Sun PD. Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):358-65.