Products > Crystallization Screens > Grid Screens > Grid Screen Sodium Chloride
Grid Screen Sodium Chloride
Applications
- Primary or secondary, Sodium chloride versus pH grid crystallization screen for biological macromolecules
Features
- A systematic grid screen varying Sodium chloride concentration versus pH
- Samples pH 4 to 9
- Samples four concentrations of Sodium chloride
- Preciase pH versus Sodium chloride screen
- pH adjusted after salt addition; 25°Celsius
- Combine reagents between rows or columns to create expanded grid screens
Description
Grid Screen™ Sodium Chloride is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.
The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Sodium chloride between pH 4.0 and 9.0.
Grid Screen Sodium Chloride evaluates Sodium chloride (HR2-219) at four concentrations (1.0, 2.0, 3.0, 4.0 M) versus six pH levels (4, 5, 6, 7, 8, 9).
Grid Screen Sodium Chloride consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salt and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Support Material(s)
HR2-219 Grid Screen Sodium Chloride Documents HR2-219 Grid Screen Sodium Chloride SDS
Grid Screen Sodium Chloride Formulation & Scoring Data
Related Item(S)
References
1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.
2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.
3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.
4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.
5. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.
