Products > Crystallization Screens > Grid Screens > Grid Screen Salt HT
Grid Screen Salt HT
Applications
- Primary or secondary, Ammonium sulfate, Sodium malonate, Sodium potassium chloride, and Sodium chloride versus pH grid crystallization screen for biological macromolecules
Features
- A systematic grid screen varying Ammonium sulfate, Sodium malonate, Sodium potassium chloride, and Sodium chloride concentration versus pH
- Samples pH 4 to 9
- Samples four concentrations of Ammonium sulfate, Sodium malonate, Sodium potassium phosphate, and Sodium chloride
- Precise pH versus Ammonium sulfate, Sodium malonate, Sodium potassium phosphate, and Sodium chloride screen
- pH adjusted after salt addition; 25°Celsius
Description
Grid Screen™ Salt HT is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.
The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Ammonium sulfate, Sodium malonate, Sodium potassium phosphate, and Sodium chloride between pH 4.0 and 9.0.
Grid Screen Salt HT combines Grid Screen Ammonium Sulfate, Grid Screen Sodium Malonate, Quik Screen and Grid Screen Sodium Chloride into a single 96 Deep Well block format. The block contains 1 ml of each reagent.
Grid Screen Salt HT consists of 96 unique reagents, each supplied as 1 milliliter aliquots in a sterile, Deep Well block format. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Support Material(s)
HR2-248 Grid Screen Salt HT Documents HR2-248 Grid Screen Salt HT SDS
Grid Screen Salt HT Formulation & Scoring Data
References
1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.
2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.
3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.
4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.
5. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.