Products > Crystallization Screens > Grid Screens > Grid Screen PEG/LiCl

Grid Screen PEG/LiCl

Applications

  • Primary or secondary, PEG & Lithium chloride versus pH grid crystallization screen for biological macromolecules

Features

  • A systematic grid screen varying Polyethylene glycol 6,000 concentration in Lithium chloride versus pH
  • Samples pH 4 to 9
  • Samples four concentrations of Polyethylene glycol 6,000 in the presence of 1.0 M Lithium chloride
  • Precise pH versus PEG and Lithium chloride screen
    • pH adjusted after salt addition; 25°Celsius
  • Combine reagents between rows or columns to create expanded grid screens

Description

Grid Screen™ PEG/LiCl is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in PEG and Lithium chloride between pH 4.0 and 9.0.

Grid Screen PEG/LiCl evaluates Polyethylene glycol 6,000 at four concentrations (0, 10, 20, 30 %w/v) in the presence of 1.0 M Lithium chloride versus six pH levels (4, 5, 6, 7, 8, 9).

Grid Screen PEG/LiCl consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity polymer, salt, and buffers. Individual reagents are available through the Hampton Research Custom Shop.



CAT NO

HR2-217

NAME

Grid Screen PEG/LiCl

DESCRIPTION

10 ml, tube format

PRICE

$124.00

Support Material(s)

Related Item(S)

References

1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.

2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.

4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

5. Expression, purification and preliminary structural analysis of the coiled-coil domain of Deinococcus radiodurans RecN. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1; 68(Pt 2): 218-221.

6. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.