Grid Screen MPD

Applications

  • Primary or secondary, (+/-)-2-Methyl-2,4-pentanediol (MPD) versus pH grid crystallization screen for biological macromolecules

Features

  • A systematic grid screen varying (+/-)-2-Methyl-2,4-pentanediol (MPD) concentration versus pH
  • Samples pH 4 to 9
  • Samples four concentrations of (+/-)-2-Methyl-2,4-pentanediol (MPD)
  • Precise pH versus (+/-)-2-Methyl-2,4-pentanediol (MPD) screen
    • pH adjusted after reagent addition; 25°Celsius
  • Combine reagents between rows or columns to create expanded grid screens

Description

Grid Screen™ MPD is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in (+/-)-2-Methyl-2,4-pentanediol (MPD) between pH 4.0 and 9.0.

Grid Screen MPD evaluates (+/-)-2-Methyl-2,4-pentanediol (MPD) at four concentrations (10, 20, 40, 65% v/v) versus six pH levels (4, 5, 6, 7, 8, 9).

Grid Screen MPD consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity polyol and buffers. Individual reagents are available through the Hampton Research Custom Shop.



CAT NO

HR2-215

NAME

Grid Screen MPD

DESCRIPTION

10 ml. tube format

PRICE

$124.00

Support Material(s)

Related Item(S)

References

1. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.

2. Crystallization of Biological Macromolecules. Alexander McPherson. pp. 271-329. Cold Spring Harbor Laboratory Press, 1999. ISBN 0-87969-527-7.

3. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.

4. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

5. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.

6. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

7. Crystallization studies of the keratin-like domain from Arabidopsis thaliana SEPALLATA 3. Samira Acajjaoui and Chloe Zubieta. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep 1; 69(Pt 9): 997-1000.