Products > Crystallization Screens > Grid Screens > Grid Screen Ammonium Sulfate

Grid Screen Ammonium Sulfate

Applications

  • Primary or secondary, Ammonium sulfate versus pH grid crystallization screen for biological macromolecules

Features

  • A systematic grid screen varying Ammonium sulfate concentration versus pH
  • Samples pH 4 to 9
  • Samples four concentrations of Ammonium sulfate
  • Precise pH versus Ammonium sulfate screen
    • pH adjusted after salt addition; 25°Celsius
  • Combine reagents between rows or columns to create expanded grid screens

Description

Grid Screen™ Ammonium Sulfate is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Ammonium sulfate between pH 4.0 and 9.0. Grid Screen Ammonium Sulfate systematically evaluates Ammonium sulfate at four concentrations (0.8, 1.6, 2.4, 3.0 M) versus 6 pH levels (4, 5, 6, 7, 8, 9).

Grid Screen Ammonium sulfate consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salt and buffers. Individual reagents are available through the Hampton Research Custom Shop.



CAT NO

HR2-211

NAME

Grid Screen Ammonium Sulfate

DESCRIPTION

10 ml, tube format

PRICE

$124.00

Support Material(s)

Related Item(S)

References

1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.

2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.

4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

5. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.