Products > Optimize Reagents > Optimize - Buffers > BIS-TRIS Buffer
BIS-TRIS Buffer
Applications
- Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
BIS-TRIS
Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2""-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C
HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 - 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 - 1.37150 at 20°C
HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 - 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 - 1.36926 at 20°C
Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.05%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na ≤0.005%
Pb ≤0.001%
SO4 ≤0.05%
Zn ≤0.0005%
Support Material(s)
HR2-781 BIS-TRIS pH 5.5 SDSCertificate Of Analysis
Related Item(S)
References
1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.
2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.
