Products > Optimize Reagents > Optimize - Buffers > BICINE Buffer
BICINE Buffer
Applications
- Crystallization grade BICINE buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
BICINE
Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4
Mr 163.17
CAS Number [150-25-4]
EC Number 205-755-1
Beilstein Registry Number 1769362
Merck 14,1201
RTECS MB9700000
MDL Number MFCD00004295
Purity ≥ 99.0%
pKa 8.3 at 20°C, 8.3 at 25°C
Useful pH Range 7.4-9.3
Titrate HR2-509 to useful pH range using NaOH
HR2-509 pH Range: 5.0 - 5.3 at 25°C
HR2-509 Measured Conductivity Range: 100.7 - 204.2 µS/cm at 25°C
HR2-509 Measured Refractive Index Range: 1.35769 - 1.35821 at 20°C
HR2-723 has been titrated to pH 9.0 using NaOH
HR2-723 Measured Conductivity Range: 23.9 - 25.0 mS/cm at 25°C
HR2-723 Measured Refractive Index Range: 1.36054 - 1.36122 at 20°C
Support Material(s)
HR2-509 BICINE 1.0 M solution SDSCertificate Of Analysis
Related Item(S)
References
1. Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. M. N. Isupov, A. A. Brindley, E. J. Hollingsworth, G. N. Murshudov, A. A. Vagin and J. A. Littlechild. Acta Cryst. (2004). D60, 1879-1882.