ADA Buffer

Applications

  • Crystallization grade ADA buffer for formulating screens or for optimization

Features

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

ADA (Buffer)

Synonyms: N-(2-Acetamido)iminodiacetic acid;
N-(Carbamoylmethyl)iminodiacetic acid
C6H10N2O5
H2NCOCH2N(CH2CO2H)2
Mr 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Beilstein Registry Number 1787181
Merck 14,147
Purity ≥ 99.0%
MDL Number MFCD00008031
PubChem Substance ID 24845005
pKa = 6.6 at 25°Celsius

Useful pH range of ADA is 5.6 - 7.5

Titrate HR2-507 to the desired pH using NaOH
HR2-817 is titrated to pH 6.5 using NaOH

HR2-507 measured Conductivity range: 23.6 - 28.6 mS/cm at 25°C
HR2-507 measured Refractive Index range: 1.35009 - 1.35044 at 20°C

HR2-817 measured Conductivity range: 42.2 - 50.4 mS/cm at 25°C
HR2-817 measured Refractive Index range: 1.36787 - 1.36913 at 20°C

ADA buffer is used in the Hampton Research MembFac crystallization kit.

Total Impurities Insoluble matter, passes filter test
Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.1%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.05%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%



CAT NO

HR2-507

NAME

0.5 M ADA

DESCRIPTION

100 mL

PRICE

$110.00

CAT NO

HR2-817

NAME

1.0 M ADA pH 6.5

DESCRIPTION

100 mL

PRICE

$119.00

Support Material(s)

Certificate Of Analysis

Related Item(S)

References

1. Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin. Arni, RK; Padmanabhan, K; Padmanabhan, KP; Wu, TP; Tulinsky, A. Biochemistry 32, 4727- 4737, 1993.

2. Structures of Three Crystals Forms of the Sweet Protein Thaumatin. Ko, TP; Day, J; Greenwood, A; McPherson, A. Acta Crystallogr D 50, 813- 825, 1994.