Make Tray Instructions

About Make Tray
Make Tray is a free, web-based program which generates both a pipetting worksheet and a plate formulation document for crystallization set ups. Make Tray allows one to enter General Information about the sample and experiment which is printed on the pipetting worksheet and the formulation document. Three different functions, Create, Reproduce, and Optimize can be selected by clicking the respective tab.

Create custom crystallization experiment.

Reproduce the formulation of a single reagent from Hampton Research crystallization screens.

Optimize a single reagent from Hampton Research crystallization screens in a 24 well format.

Make Tray was originally created by Max Nanao and updated to include a number of enhancements, including the Reproduce and Optimize functions by Hampton Research, and Michael Carney and Tom Hanberg of Skyhound Internet.

Instructions for using Create in Make Tray

Create custom crystallization experiments

Number of Reagents

Enter the Number of Reagents and click Create Reagent. The Reagent Number fields will appear below the General Information section of the page. Between 1 to 99 additions can be accommodated with Make Tray.

General Information
Information such as Experiment ID, Sample Name, Your Name, and Sample Concentration, is entered under the section titled "General Information". This required information will be printed on the pipetting worksheet and formulation sheet. General Information are required fields and Make Tray will only display results in a new tab if all fields are completed.

Setting up your Plate Size
Enter Tray Number. Unique tray numbers can be used to further identify the experiment. Enter
the number of reservoirs in the X direction (typically printed 1, 2, 3, 4 ... on the plate) and the Y
direction (typically printed A, B, C, D ... on the plate). The plate size can be customized for any number of wells. A 24 well plate would be created by entering 6 for X and 4 for Y. A 96 well plate would be created by entering 12 for X and 8 for Y. To generate a set of dilutions or pipetting instructions for a single reservoir, enter 1 for X and 1 for Y. Enter the desired Reservoir Volume in microliters. The reservoir volume is adjustable but must be the same for each well. Setting up your Plate Size are required fields and Make Tray will only display results in a new tab if all fields are completed.

Reagent Name and Reagent Stock Concentration
Enter the reagent name and the stock concentration and select the concentration units (M and %). For each reagent, select one of the 11 available dilution options by clicking the appropriate radio button. The dilution option can be the same or different for each of the reagents.

Choose one of the following options to construct your grid for Reagent “?”
For each reagent, select one of the eleven available dilution options by clicking the appropriate radio button. The dilution option can be the same or different for each of the reagents.

Set of concentrations in the "X" direction: List the desired final reservoir concentration,
separating each entry with a space. For example, enter 1 1.2 1.4 1.6 1.8 2.0 to have this be the
final concentration of a reagent across the 6 columns.

Set of concentrations in the X direction in row number: List the desired final reservoir concentration, separating each entry with a space. For example, enter 1 1.2 1.4 1.6 1.8 2.0 and choose number B to have this be the final concentration of a reagent across the 6 columns in row B.

Set of concentrations in the "Y" direction: List the desired final reservoir concentration,
separating each entry with a space. For example, enter 1 1.2 1.4 1.6 to have this be the final
concentration of a reagent down the 4 rows.

Set of concentrations in the "Y" direction in column number: List the desired final reservoir concentration, separating each entry with a space. For example, enter 1 1.2 1.4 1.6 and choose in column number 6 to have this be the final concentration of a reagent down the 4 rows in column 6.

Increase in the "X" direction from the lowest value of "?" in steps of "?": Enter 1 in the first field and 0.3 in the second field to have the reagent concentration be 1 in column 1, 1.3 in column 2, 1.6 in column 3, 1.9 in column 4, 2.2 in column 5 and 2.5 in column 6.

Increase in the "Y" direction from the lowest value of "?" in steps of "?": Enter 10 in the first field and 5 in the second field to have the reagent concentration be 10 in row 1, 15 in row 2, 20 in row 3 and 25 in row 4.

Decrease in the "X" direction from the highest value of "?" in steps of "?": Enter 2 in the first field and 0.2 in the second field to have the reagent concentration be 2 in column 1, 1.8 in column 2, 1.6 in column 3, 1.4 in column 4, 1.2 in column 5 and 1.0 in column 6.

Decrease in the "Y" direction from the highest value of "?" in steps of "?": Enter 20 in the first field and 3 in the second field to have the reagent concentration be 20 in row 1, 17 in row 2, 14 in row 3 and 11 in row 4.

Constant concentration of "?" throughout the entire tray: Enter 0.2 to have a concentration of 0.2 in each and every well or reservoir.

Constant concentration of "?" in column number "?" (starting from the left): Enter 0.5 in the first field and 4 in the second field to have the first three columns of the plate contain none of the reagent in question, and then starting with column 4, have a concentration of 0.5.

Constant concentration of "?" in row number "?" (starting from the top): Enter 0.01 in the first field and 3 in the second field to have the first two rows of the plate contain none of the reagent in question, and then starting with row 3, have a concentration of 0.01.

Remove Reagent
Click to remove a reagent.

Add Reagent
Click to add a reagent.

Make Tray
Click "Make Tray" to create the pipetting worksheet and formulation sheet. Make Tray results open in a new tab. Some browsers with pop-up blockers may prevent the results from opening. Please adjust your pop-up blocker accordingly.

Instructions for using Reproduce in Make Tray

Reproduce the formulation of a single reagent from Hampton Research crystallization screens

Choose a Crystallization Screen and Reagent
Choose a Crystallization Screen and a Reagent from the drop-down menus and click on Reproduce Reagent. The reagents necessary to reproduce the crystallization screen condition will appear below the General Information section.

General Information
Information such as Experiment ID, Sample Name, Your Name, and Sample Concentration, is entered under the section titled "General Information". This required information will be printed on the pipetting worksheet and formulation sheet. General Information are required fields and Make Tray will only display results in a new tab if all fields are completed.

Setting up your Plate
Enter Tray Number. Unique tray numbers can be used to further identify the experiment. Setting Up Your Plate are required fields and Make Tray will only display results in a new tab if all fields are completed. Enter the desired Reservoir Volume in microliters.

Make Tray
The Complete, Ready to Use Custom Shop reagent to reproduce the selected Crystallization Screen and Reagent, along with the individual Optimize Reagents to formulate the selected Crystallization Screen and Reagent are displayed and can be added to the cart for purchase.

Also displayed are the Reagent Name, Reagent Stock Concentration and the appropriate dilution function for each of the reagents to reproduce the formulation of the single reagent. To create the pipetting worksheet and formulation sheet for the selected Crystallization Screen and Reagent identical to the kit formulation, click "Make Tray". Make Tray results open in a new tab. Some browsers with pop-up blockers may prevent the results from opening. Please adjust your pop-up blocker accordingly.

Optionally, one may Remove Reagent, Add Reagent, change the Reagent Name, Change the Reagent Stock, and dilution function to modify the reagent. For details, refer to Instructions for using Create in Make Tray. To create the pipetting worksheet and formulation sheet for a modified reagent, make the desired changes, then click "Make Tray". Make Tray results open in a new tab. Some browsers with pop-up blockers may prevent the results from opening. Please adjust your pop-up blocker accordingly.

Instructions for using Optimize in Make Tray

Optimize a single reagent from Hampton Research crystallization screens in a 24 well format

Choose a Crystallization Screen and Reagent
Choose a Crystallization Screen and a Reagent from the drop-down menus and click on Optimize Reagent. The reagents necessary to optimize the crystallization screen condition will appear below the General Information section.

General Information
Information such as Experiment ID, Sample Name, Your Name, and Sample Concentration, is entered under the section titled "General Information". This required information will be printed on the pipetting worksheet and formulation sheet. General Information are required fields and Make Tray will only display results in a new tab if all fields are completed.

Setting up your Plate
Enter Tray Number. Unique tray numbers can be used to further identify the experiment. Click “Optimize Reagent”. Setting Up Your Plate are required fields and Make Tray will only display results in a new tab if all fields are completed. Enter the desired Reservoir Volume in microliters.

Make Tray
The individual Optimize Reagents to formulate the selected Crystallization Screen and Reagent optimization are displayed and can be added to the cart for purchase.

Also displayed are the Reagent Name, Reagent Stock Concentration and the appropriate dilution function for each of the reagents to formulate a 24 well optimization screen. To create the pipetting worksheet and formulation sheet for the selected Crystallization Screen and Reagent optimization, click "Make Tray". Make Tray results open in a new tab. Some browsers with pop-up blockers may prevent the results from opening. Please adjust your pop-up blocker accordingly.

Optionally, one may Remove Reagent, Add Reagent, change the Reagent Name, Change the Reagent Stock, and dilution function to modify the reagent. For details, refer to Instructions for using Create in Make Tray. To create the pipetting worksheet and formulation sheet for a modified optimization, make the desired changes, then click "Make Tray". Make Tray results open in a new tab. Some browsers with pop-up blockers may prevent the results from opening. Please adjust your pop-up blocker accordingly.