Heliful Link Banner Image


Make Tray Instructions

About MakeTray
MakeTray is a free, web based program which generates both a pipetting worksheet and a plate
formulation document for crystallization set ups. MakeTray allows one to enter general
information about the sample and experiment which is printed on the pipetting worksheet and
the formulation document. The plate size can be customized for any number of wells, so
MakeTray works for 24, 48 and 96 well plates. The reservoir volume is adjustable but must be
the same for each well. A reservoir can include up to 12 unique chemicals or additions.
MakeTray is especially useful for optimization experiments.  MakeTray was created by Max
Nanao and reprogrammed by Michael Carney of Skyhound Internet.

Instructions for using MakeTray

General Information
Information such as Experiment ID, Sample Name, Your Name and Sample Concentration is
entered under the section entitled "General Information". This information will be printed on
the pipetting worksheet and formulation sheet.


Setting Up The Plate Size
Enter Tray Number. Unique tray numbers can be used to further identify the experiment. Enter
the number of reservoirs in the X direction (typically printed 1, 2, 3, 4 ... on the plate) and the Y
direction (typically printed A, B, C, D ... on the plate). A 24 well plate would be created by
entering 6 for X and 4 for Y. A 96 well plate would be created by entering 12 for X and 8 for Y. To
simply generate a set of dilutions or pipetting instructions for a single reservoir, enter 1.


Reagent Number 1-12
Between 1 to 12 additions can be accommodated with MakeTray. Enter the reagent name and
the stock concentration and select the concentration units. For each reagent, select the desired
dilution option. The dilution option can be the same or different for each of the reagents.

Set of concentrations in the "X" direction: List the desired final reservoir concentration,
separating each entry with a space. For example, enter 1 1.2 1.4 1.6 1.8 2.0 to have this be the
final concentration of a reagent across the 6 columns in a VDX Plate.


Set of concentrations in the "Y" direction: List the desired final reservoir concentration,
separating each entry with a space. For example, enter 1 1.2 1.4 1.6 to have this be the final
concentration of a reagent down the 4 rows in a VDX Plate.


Increase in the "X" direction from the lowest value of "?" in steps of "?": Enter 1 in the first field
and 0.3 in the second field to have the reagent concentration be 1 in column 1, 1.3 in column 2,
1.6 in column 3, 1.9 in column 4, 2.2 in column 5 and 2.5 in column 6.


"Increase in the "Y" direction from the lowest value of "?" in steps of "?": Enter 10 in the first
field and 5 in the second field to have the reagent concentration be 10 in row 1, 15 in row 2, 20
in row 3 and 25 in row 4.


Constant concentration of "?" throughout the entire tray: Enter 0.2 to have a concentration of
0.2 in each and every reservoir of the tray.


Constant concentration of "?" in column number "?" (starting from the left): Enter 0.5 in the first
field and 4 in the second field to have the first three columns of the plate contain none of the
reagent in question, and then starting with column 4, have a concentration of 0.5.


Constant concentration of "?" in row number "?" (starting from the top): Enter 0.01 in the first
field and 3 in the second field to have the first two rows of the plate contain none of the reagent
in question, and then starting with row 3, have a concentration of 0.01.


Build Tray
Click "Clear fields" to clear all the fields for the desired reagent number. Click "Build Tray" to
have MakeTray create the pipetting worksheet and formulation sheet. "Clear fields" and "Build
Tray" are located at the end of each "Reagent Number" area for convenience.