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Crystallography related career opportunities, post docs and related positions in the crystallography community. If you wish to place your job opportunity on the Hampton Research website, submit the details to tech@hrmail.com.


Postdoctoral fellowship available to study the structure and evolution of viral proteins at Linköping University, Sweden

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Description:

Postdoctoral fellowship to study structural and functional evolution of viral proteins

The von Castelmur lab at the Department of Physics, Chemistry and Biology (IFM) and part of the Wallenberg Center for Molecular Medicine at Linköping University, Sweden, is looking to recruit a postdoctoral fellow (2-year fellowship, funded by Knut and Alice Wallenberg foundation) to study the evolutionary relationship and functional repurposing of human proteins acquired by picornaviruses (through horizontal gene transfer). The overall goals of this particular project is to continue the structural and functional characterisation of these viral proteins already begun in the lab. The project will involve molecular biology, biochemistry and biophysics in combination with structural biology techniques (in house access to a variety of techniques through ProLinC). Therefore, we seek highly motivated individuals with practical experience and expertise in cloning and protein production (bacterial expression, purification, characterization), structural biology (experience in at least one technique is required) and an interest in learning new methods and skills.

Qualifications and requirements to applicants:

  • Postdoctoral fellowship may be granted only to non-Swedish citizens with a PhD or equivalent acquired in a country other than Sweden.
  • The applicant must have or be about to receive a doctoral degree in a subject relevant to the research project (e.g. structural biology, molecular biology, biochemistry, biophysics, chemistry, or a related area) and needs to be passionate about research. Problem solving ability and creativity are essential.
  • Documented ability to write scientific manuscripts (one or more first author papers from Ph.D. and postdoctoral studies will be highly valued)
  • Excellent English interpersonal, communication and writing skills
  • Ability to operate both independently but also as a team player in a multi-disciplinary Environment

Application

The application should consist of the following:

  1. A short (maximum 1 A4 page) cover letter, explaining the motivation to apply for the position.
  2. The CV of the applicant, including a list of published peer-reviewed articles.
  3. Statement of Research Interests, max 2 pages
  4. Copies of the PhD diploma (or equivalent).
  5. Contact details for 2-3 references, one of which should be your PhD supervisor.

The duration of the fellowship is 2 years. Your application should be written in English and preparedas a single package in PDF format, to be submitted to eleonore.von.castelmur@liu.se. Make sure to use the subject line: “application for postdoctoral fellowship 2021” in the application email. Deadline for application is 31st of July 2021. The top ranked candidates will be contacted within three weeks from the closing date for an interview. Starting date according to agreement, but ideally starting as soon as possible. For more information, contact Dr. Eleonore von Castelmur.

Webpage: https://liu.se/en/research/von-castelmur-lab

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[Post Date: June 18, 2021]


Opening for Postdoctoral Fellow / Habilitant in Structural Biology at Ruhr-University Bochum

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Description:

We have an opening in our group for an enthousiastic Postdoc/Habilitant.

The Hofmann group is located at the Ruhr-University in Bochum in the metropolitan Ruhrgebiet in the center of Germany.

Projects in the group target biological hydrogen production, photosynthesis, membrane transport and plant enzymes utilizing biochemical, biophysical and structure based methods.

You are expected to be taking responsibility for one of the project areas.

If you plan to build up your own research profile, we strongly support you in applying for individual project funding.

The position has a medium term funding perspective, if you plan to pursue a habilitation on your way to your own independent research position. Both faculty and university have mentoring programs assisting you as well.

Due to some teaching at the undergrad level, at least basic command of german is desirable.

The position can be filled immediately, but don't hesitate to contact me, if your current position is still ongoing.

For details see our webpage (https://www.bioxtal.rub.de) or drop me (Prof. Dr. Eckhard Hofmann) an email at eckhard.hofmann@rub.de.


[Post Date: June 15, 2021]


Postdoc Structural Studies of Ion Channels at the University of Chicago

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Description:

A full-time position for a postdoctoral researcher is available in the Department of Biochemistry and Molecular Biology at the University of Chicago to pursue structural studies on a novel ion channel using X-ray crystallography and cryo-electron microscopy, in combination with various biochemical, electrophysiological and computational techniques.

 

The ideal candidate will be an independent and highly motivated researcher with a strong background in membrane protein biochemistry and advanced techniques in protein crystallography.

 

Funding for the position is secured for at least 4 years, with opportunities for extension and, depending on performance, promotion to a "Staff Scientist" level position, allowing for considerable autonomy to develop independent research projects.

 

Please contact Benoit Roux roux@uchicago.edu directly if you are interested or know of a suitable interested candidate.  The search will continue until the position is filled.


[Post Date: June 14, 2021]


Postdoc Neiditch Lab at Rutgers University - New Jersey Medical School

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Description:

An NIH-funded postdoctoral position is available in the Neiditch lab at Rutgers University - New Jersey Medical School to develop new anti-TB drugs that inhibit cell wall biosynthesis and respiration. In addition to a Ph.D., the ideal candidate will have a background in macromolecular X-ray crystallography, cryo-EM, biochemistry, protein purification, and molecular biology. Candidates with demonstrated success in another field and a strong commitment to learning X-ray crystallography and cryo-EM will also be considered. A working knowledge of bacterial genetics is a plus. The ability to communicate ideas and work collaboratively as a member of a highly motivated team is essential.

Please submit your CV to matthew.neiditch@rutgers.edu with the subject line Postdoctoral Position Applicant.


[Post Date: June 14, 2021]


Postdoc Biochemistry/Molecular Biology

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Description:

We have a Postdoctoral position in Biochemistry/Molecular Biology ”Regulation of microbial ribonucleotide reductases” in the department of Biochemistry and Biophysics, Stockholm University.  Please see the attached document.  Applications will be reviewed on a continuous basis until the position is filled.

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[Post Date: June 14, 2021]


Structural Biology & Biophysics Scientist (PROTAC drug discovery)

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Description:

Unit: 
Biological Chemistry and Drug Discovery
Job Reference: 
SLSC0882
Closing Date: 
Wednesday, June 23, 2021
Salary: 
Grade 7 (£32,817 - £40,322)

The School of Life Sciences at the University of Dundee is a world-class academic institution with a reputation for the excellence of its research, its high quality teaching and student experience, and the strong impact of its activities outside academia. With 900 staff from over 40 countries worldwide the School provides a dynamic, multi-national, collegiate and diverse environment with state-of-the-art laboratory, technology and teaching facilities.

We are seeking a highly motivated scientist with research experience in structural biology and biophysics to join a multi-disciplinary drug discovery team based in a new Centre for Targeted Protein Degradation (CeTPD) within Dundee’s School of Life Sciences (https://www.dundee.ac.uk/stories/new-protein-degradation-centre-lead-drug-discovery-revolution).

The team is engaged in an exciting collaboration on proteolysis-targeting chimeras (PROTAC) drug discovery between the laboratories of Professor Alessio Ciulli (http://www.lifesci.dundee.ac.uk/groups/alessio-ciulli) and Boehringer Ingelheim. Our mission is to discover novel small-molecule degraders to treat cancer and other human diseases with serious unmet medical needs. The scientist will be expected to boost the structure and biophysics guided approaches the team are taking to accelerate translation of research in this field and to develop degrader drug candidates.

The post is available to start immediately. Salary will depend on experience and will be up to £40,322. This is a unique opportunity to gain experience in world-leading industry-style drug discovery within an academic setting. The post may also provide opportunities for project leadership and to carry out a short work placement at the collaborator research site. The position is available until 31st December 2023 in the first instance.

Your priorities will include:

  • Characterise the binding of small molecule bifunctional degraders to their target protein and E3 ligases using X-ray crystallography, NMR spectroscopy and/or cryo-electron microscopy (cryo-EM) amongst other structural methods.
  • Design constructs and express and purify proteins to enable compound evaluation via a broad spectrum of biophysical and biochemical techniques.
  • Execute and optimize binding assays in vitro to profile novel compounds (including but not limited to proximity and fluorescence-based assays, ITC, and SPR)
  • Carry out data analysis and relevant quality assurance protocols
  • Develop team science across the collaboration, working with colleagues across disciplines to enable breakthroughs.
  • Present findings to the collaboration team and externally at international conferences
  • Report directly to the Collaboration Leader (Will Farnaby) and to the Principal Investigator (Alessio Ciulli)

Who we’re looking for:

  • Ph.D. in structural biology, biophysics, biochemistry, chemical biology or a related discipline
  • Highly experienced in structural biology and biophysics, with an emphasis on structure-based drug design.
  • Strong background in molecular biology (construct design, cloning etc.), protein expression, purification and characterisation
  • Experience with either protein crystallization, X-ray data collection (in-house and at synchrotrons), structure solution and model refinement, or protein NMR spectroscopy, or cryo-EM.
  • Strong interpersonal, communication and presentation skills

Preferred skills:

  • Outstanding academic or industrial track records
  • Experience working in drug discovery teams.
  • Strong data science and computational skills
  • Exceptional time management, organisational and self-motivational skills

Appointment on the Grade 7 salary scale is dependent upon you having been awarded a PhD. An appointment may be considered if you are shortly expected to be awarded a PhD.

Informal enquiries may be made to Professor Alessio Ciulli, e-mail: a.ciulli@dundee.ac.uk

Additional Information:

We are one of the UK’s leading universities – internationally recognised for our expertise across a range of disciplines and research breakthroughs in multiple areas, including science, medicine and engineering, amongst many others. Conveniently located on the banks of River Tay, our main city-centre campus is at the heart of Dundee - an up-and-coming, friendly, compact and affordable city with a rich heritage in design and technology. Just a short walk from the V&A Museum of Design Dundee, we’re close to both the train and bus stations. We also have campuses at Ninewells Hospital and in Kirkcaldy which are easily accessible via local transport links.

The new Protein Degradation Centre is located in a beautiful setting overlooking the estuary of the River Tay. It is part of a growing Life Sciences Innovation District, an ecosystem of organisations and people within the life sciences sector in Dundee driving innovation and local economic growth. The Centre is embedded within the University of Dundee’s School of Life Sciences, one of the premier research centres in the world recently assessed as the top UK University for Life Sciences research in the 2014 Research Excellence Framework (REF). The University of Dundee main city-centre campus is at the heart of Dundee - an up-and-coming, friendly, compact and affordable city with a rich heritage in design and technology. Just a short walk from the V&A Museum of Design Dundee, we’re close to both the train and bus stations. http://www.lifesci.dundee.ac.uk/

As an internationally diverse institution, we welcome job applicants from all countries and nationalities. The School of Life Sciences is proud to employ staff from over 40 different nations. 

The diversity of our staff and students helps to make the University of Dundee one of the top universities in the UK. Family friendly policies, staff support networks for BME and LGBT staff, membership of Athena Swan and Stonewall, as well a full range of disability services, create an enjoyable and inclusive place to work.

The Closing Date is midnight on 23rd June 2021.


[Post Date: June 11, 2021]


Postdoc University of California Irvine

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Description:

A funded postdoctoral position in structural biology is available immediately in the laboratory of Professor Celia Goulding at the University of California, Irvine.  Our studies focus on the structural biology and biochemistry of Mycobacterium tuberculosis iron/heme uptake and bacterial contact-dependent growth inhibition.  Potential projects will utilize X-ray crystallography, electron microscopy and biochemical studies.  A Ph.D. in biochemistry or molecular biology with experience in protein crystallography is preferred.  This position is an excellent opportunity to develop broad skills in structure determination, kinetics, spectroscopy, genetics, and molecular biology while working in an energetic, interdisciplinary environment 7 miles from the beautiful Southern California coast.

Please refer to http://faculty.sites.uci.edu/celiagoulding and apply to celia.goulding@uci.edu.


[Post Date: June 10, 2021]


Structural Biology Postdoc at Princeton University

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Description:

An NIH-funded postdoctoral research position is available immediately in the Hughson lab at Princeton University. Our group’s research focuses on the remarkable protein machinery that generates the interior architecture of eukaryotic cells by orchestrating the formation and fusion of cargo-carrying transport vesicles. Our recent discoveries lay the foundation for exciting new projects studying how membrane tethering proteins work together with membrane fusion proteins to ensure accurate and efficient cargo delivery. This work will combine structural techniques (cryoEM, crystallography) with mechanism-directed functional studies. If this sounds interesting, please apply to join our team by sending your CV, a cover letter describing your research interests, and contact information for three references to  hughson@princeton.edu .

 

Princeton University is renowned for its scientific excellence and collegial, interactive environment. Located in Princeton, New Jersey, a vibrant community of about 30,000 people, it is just a quick train ride away from New York City and Philadelphia. Princeton University is an equal opportunity employer, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veteran status, or any other characteristic protected by law.


[Post Date: June 10, 2021]


Principal Beamline Scientist

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Description:

About Us

Diamond Light Source is the UK’s national 3rd generation synchrotron science facility. Life sciences is a significant strength at Diamond with structural biology being a major driver. Diamond currently has 7 world-class beamlines dedicated to macromolecular crystallography (MX) covering a diverse and unique set of capabilities. MX is complemented by dedicated beamlines for BIOSAXS, CD, X-ray imaging and spectroscopy, IR-micro-spectroscopy and the UK’s national Cryo-EM centre (eBIC), together creating a unique environment for integrative structural biology research.

The MX group at Diamond established the first dedicated synchrotron-based labs for X-ray fragment screening (XChem) and membrane proteins (MPL). In addition, the UK XFEL Hub provides a focus for XFEL work, engaging UK users in serial crystallography experiments around the world and directs UK developments at the SPB/SFX instrument at the European XFEL. The MX group has a strong software development programme which includes development of the DIALS integration software package, ISPyB/SynchWeb and automation pipelines.

About the Role

We now have an opportunity for a Principal Beamline Scientist (PBS) with a keen interest in beamline development, automation and/or structural biology, to join the macromolecular crystallography (MX) leadership team within Diamond to guide and develop the highly automated I03 beamline.

I03 is a state-of-the-art macromolecular crystallography beamline at Diamond, uniquely being capable of collecting data from crystalline samples up to biological containment level 3. It has a broad energy range and is equipped with a state-of-the-art detector (Eiger2 XE 16M) and very high capacity, rapid sample changer (BART). To date the team has led many of the developments for cryogenic sample automation and automated data collection.

In the role of PBS you will manage a small team of scientists to establish a programme which furthers the capabilities of I03 and MX at Diamond and contribute to the development and operation of the I03/MX group international user programme.

The post is underpinned by funding for a post-doctoral research associate to support research and development within the group subject to a suitable case being made. Furthermore, Diamond has a successful PhD programme and is partnered with a number of doctoral training centres across the UK providing the opportunity for PhD studentships. You will have access to well-equipped laboratories for structural and cellular biology at Diamond and within the Research Complex at Harwell.

Instrumentation and software development is facilitated by dedicated support from Diamond’s computing, engineering and scientific software groups as well as strategic partnerships with CCP4 and the recently established Rosalind Franklin Institute amongst others. Candidates are encouraged to apply for external grant funding through national and international funding agencies to take full advantage of the stimulating and exciting research environment at Diamond.

Looking to the future, the expected Diamond-II upgrade programme of machine and beamlines presents a unique opportunity for the post holder to develop advanced methodologies in automation for MX and a complementary user programme for I03 for structural biology studies, including where appropriate exploiting the beamlines unique biological containment level 3 capability.

About You

You will hold appropriate first and higher degrees or demonstrate significant equivalent experience.

You will be a highly talented structural biologist or methods development expert, with expertise in macromolecular crystallographic techniques as applied at synchrotron beamlines, and a broad outlook in structural biology.

Experience of beamline instrumentation, experiment automation and/or sample environment equipment, biological containment environments, will benefit your application.

You will demonstrate skills in managing a small team, as well as a managing a budget and other resources for research and operations and upgrade projects.

You will have demonstrated ability to develop a research programme, and to lead significant projects.

You will display excellent interpersonal, communication and presentational skills, to interact effectively with staff and stakeholders at all levels.

Benefits

Diamond offers an exceptional benefits package to support staff in achieving a positive work/life balance. This includes 25 days annual leave plus 13 days of statutory and company holidays, along with flexible working hours and an excellent pension scheme. Staff also have access to a range of amenities on site including a nursery, cafes, a subsidised restaurant and sports and leisure facilities. A relocation allowance may also be available where applicable.

To Apply

Please use the online application process to apply and tell us why you believe you are suitable for this role.


[Post Date: June 10, 2021]


Senior Scientist/Scientist, Structural Biology

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Description:

We are seeking an experienced and motivated structural biologist to join the Protein Sciences and Lead Discovery team at Kymera Therapeutics.  As part of a dynamic team, the successful candidate will provide critical structural support (both x-ray crystallography and cryo-electron microscopy) for the identification and optimization of ligands to novel and proprietary E3 ligases as well as “undruggable” proteins of interest and will both conduct internal and drive external experiments to advance projects toward their goals. Kymera is advancing the field of targeted protein degradation, accessing the body’s innate protein recycling machinery to degrade rather than inhibit dysregulated, disease-causing proteins. This position will also play an important role in the progression of these binders to degraders. The successful candidate will work in a fast-paced and highly matrixed discovery environment requiring scientific rigor, organized and independently driven work, and exceptional attention to detail. 

Kymera Therapeutics is a clinical stage biotechnology company focused on advancing the field of targeted protein degradation (TPD), a transformative new approach to address previously intractable disease targets. Kymera’s Pegasus™ TPD platform harnesses the body’s natural protein recycling machinery to degrade disease-causing proteins, with a focus on undrugged nodes in validated pathways currently inaccessible with conventional therapeutics. Our mission is to discover, develop, and commercialize transformative therapies while leading the evolution of targeted protein degradation. Kymera’s goal is to become a fully integrated biopharmaceutical company with a pipeline of novel medicines, with an initial focus on immune-inflammatory diseases and oncology.

In 2021, we plan to continue to grow and strengthen our organizational capabilities to deliver on the potential of inventing a new class of protein degrader medicines for patients. Kymera is rapidly advancing three programs in clinical studies this year. We are also actively advancing a broad pipeline of preclinical programs across a wide variety of diseases, both internally and in collaboration with our partners Sanofi and Vertex Pharmaceuticals.

Responsibilities:

  • As a member of the protein sciences and lead discovery team, lead protein sciences and structural biology efforts to identify, validate, and fully characterize small molecule ligands and progress to targeted protein degraders.
  • Contribute to the strategy for bringing novel E3 ligase and oncology/immunology targets to the Kymera portfolio.
  • In collaboration with chemistry, biology, and platform teams, characterize binary and ternary complex formation and establish SAR to rapidly drive the hit finding, hit-to-lead, and lead optimization process.
  • Deliver data, contribute to critical data evaluation, and provide strong discipline leadership to project teams.
  • Augment our toolbox of structural biology techniques to address the specific needs of targeted protein degradation drug discovery.

Qualifications:

  • PhD in Structural Biology or related discipline.
  • 1-3 years of biotech/pharma industry experience with a track record of hit-finding, hit-to-lead, and lead optimization towards delivering small molecule development candidates is a plus.
  • Extensive knowledge of state-of-the-art protein structural biology techniques including all aspects of construct design, expression, purification, characterization, crystallization, structure determination and analysis.
  • Experience in single particle cryo-EM workflow including grid freezing and screening, data collection and processing, three-dimensional reconstruction and model building.
  • General knowledge of signaling pathways, protein homeostasis, and regulation of gene expression in a diverse disease context. Experience in targeted protein degradation or molecular glues a plus. 
  • Excellent presentation skills (written and oral) that enhance team-based delivery of results.
  • Ability to work independently and effectively within timelines and to collaborate in a multidisciplinary environment.
  • Skills in managing multiple projects and capability to prioritize.


[Post Date: June 10, 2021]


Associate Researcher Position in the KU Protein Structure Laboratory

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Description:

The Protein Structure Laboratory (PSL) at the University of Kansas (KU) is seeking a full-time Associate Researcher to assist with various structural biology projects.  As a core laboratory, the PSL collaborates with a diverse range of investigators to obtain structures of their proteins of interest using X-ray crystallography and delivers dozens of structures annually.  Additionally, the PSL manages the structural biology efforts for extramurally funded research projects particularly aimed at inhibitor development.       


[Post Date: June 8, 2021]


Postdoctoral Fellow Position at UT Southwestern Medical Center

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Description:

A Postdoctoral Fellow position is immediately available for a highly motivated individual in Dr. Xin Liu’s group at UT Southwestern Medical Center. Research in the Liu Lab is focused on structure and function of large macromolecular assemblies involved in formation of heritable high-order chromatin structure. To achieve the research goal, the Liu lab leverages a combination of advanced tools, including biochemical reconstitution (e.g. protein-protein, protein-nucleosome, or protein-non-coding RNA complexes), X-ray crystallography, cryo-EM, proteomics, and genomics by next-generation sequencing. The Liu lab also routinely uses a variety of cancer cells and pluripotent stem cells to perform structure-based functional studies. Additional information is available here: http://profiles.utsouthwestern.edu/profile/127166/xin-liu.html 

Applicants with demonstrated expertise in structural biology and in particular cryo-EM, protein and nucleic acid biochemistry, or chromatin biology are encouraged to apply.  Candidates must hold a Ph.D. and/or M.D. degree with first author publications and should submit a CV and contact information of 2-3 references to the PI by e-mail at: xin.liu@utsouthwestern.edu  


[Post Date: June 7, 2021]


Protein Crystallisation Position at Vernalis Research, Cambridge, UK

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Description:

Scientist, Crystallisation

Full Time/Permanent

At Vernalis, we develop and apply fragment and structure-based methods to solve problems in drug discovery and generate development candidates against targets in oncology, neurodegeneration, anti-infectives and inflammation. 

We have a new, full-time lab-based opportunity for a Scientist to join our biophysics group, located at our Cambridge Research site. 

Working in collaboration with an integrated team of protein scientists, NMR spectroscopists and Crystallographers, your primary role will be the discovery and optimisation of protein crystals for X-ray data collection and analysis, supporting multiple projects ranging from target validation to lead optimisation. 

Key Responsibilities

  • Protein crystallisation experiments across multiple projects including:
    • Protein sample handling
    • Crystallisation screening using robotics (mosquito) and manual techniques.
    • Crystal optimisation, including soaking, co-crystallisation, harvesting and crystal cryo-protection.
  • Data collection using both in house X-ray and external synchrotron facilities.
  • Follow standard experimental protocols (SEPs).
  • Interpret and communicate results to project leaders and project teams.
  • Maintain accurate experimental records using electronic notebooks.
  • Undertake duties as necessary to support wider group and ensure the efficient running of the labs.

About the candidate

This post would suit someone with a practical understanding of protein biochemistry and laboratory-based research experience, preferably gained within a drug discovery environment.  Applications will be considered from graduates with a BSc/MSc in Biochemistry, Biophysics, Chemistry (Chemical Crystallography), Molecular Biology or other related discipline, as well as candidates with an equivalent level of work experience, for example, operating as a Lab Technician.

In addition, you will have:

  • Aptitude for practical laboratory skills including sample handling, pipetting, lab safety, making up stock solutions, buffers etc.
  • Good general knowledge of IT software systems including MS Office applications.
  • An excellent attention to detail.
  • Strong communication skills (written and verbal).
  • Can be relied on to complete work to time and specification.
  • Team player.  Assists others and collaborates across disciplines.
  • Ability to organise work effectively and manage time to work on multiple projects and/or changing priorities.

Desirable attributes include:

  • Familiarity with biophysical methods such as SPR, ITC and NMR.
  • Liquid handling techniques and robotics.
  • Desire to continually look for ways to improve processes.
  • Presentation skills.

To apply, send your CV and a covering email to hr@vernalis.com

All applicants must be eligible to work and live in the UK.


[Post Date: June 7, 2021]


Postdoc RNA-protein Structural Biology / Biochemistry at University of Delaware

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Description:

Postdoctoral Researcher - Structure & Function of RNA-Modifying Enzymes

The Mugridge lab (mugridgelab.org) in the Department of Chemistry & Biochemistry at the University of Delaware is seeking a postdoctoral researcher to join our team. Our lab is focused on a mechanistic, structure-level understanding of how cellular RNA-modifying enzymes control diverse chemical modifications on mRNA and tRNA to affect RNA structure, processing, translation, and stability, and impact human disease. We are looking for an enthusiastic, self-motivated postdoc to lead a project studying the biochemistry and structural biology of mRNA-modifying enzymes; this project is fully NIH-funded with the flexibility to explore different scientific directions and ideas.

Preferred Candidates

We welcome applications from scientists of all backgrounds who are excited about understanding how protein-protein and protein-RNA interactions regulate RNA modification and function. The ideal candidate will have a PhD in biochemistry, structural biology, molecular biology, or a related field, and have experience with protein expression and purification from bacterial and eukaryotic expression systems, in vitro and/or cell-based biochemical assays, and macromolecular structural and/or biophysical techniques. Applicants with experience studying protein-nucleic acid interactions are particularly encouraged to apply. The successful applicant will also have a strong scientific record, excellent communication skills, ability to work independently and as part of team, and willingness to help mentor junior scientists. 

 

Our Lab

The Mugridge lab combines techniques from biochemistry, structural biology, biophysics, and chemical biology to determine the structural and molecular mechanisms that regulate the dynamic chemical modification landscape of RNA, or the ‘epitranscriptome’. Our lab houses brand new instrumentation for macromolecular protein function and structure work including an AKTA Pure 25M FPLC, Agilent 1260 Infinity II bio-inert UHPLC with iQMS mass spectrometer, Zeiss V20 stereomicroscope and other equipment for protein crystallography, and new infrastructure to support diverse biochemical and molecular biology techniques. We are part of a multidisciplinary NIH COBRE center at UD and have ready access to state-of-the-art core facilities including proteomics/mass spectrometry, protein crystallography, NMR, cell imaging and microscopy, and DNA/RNA sequencing cores. The University of Delaware is an R1 research University located in the heart of the mid-Atlantic region, nearby to Philadelphia, Baltimore, coastal beaches, and within easy driving distance of NYC and Washington DC.

Apply or ask!

To apply, email Jeff (mugridge@udel.edu) a single PDF with a one-page cover letter and a CV including publication record, brief descriptions of previous research accomplishments and techniques, and the names and contact information for three references. Applications will be evaluated on a rolling basis, so please apply ASAP. If interested, feel free to email Jeff with any questions!


[Post Date: June 4, 2021]


Postdoc University of Texas Health, San Antonio, Texas, USA

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Description:

Postdoctoral Fellow position at UT Health School of Medicine, San Antonio, Texas

The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at the University of Texas Health at San Antonio is seeking an enthusiastic Postdoctoral Fellow to study structures and function of RNA modification and chromatin remodeling complexes. We use structural biology (X-ray, NMR, cryo-EM) and cell-based methods to address basic mechanistic questions in viral pathogenesis and pediatric cancers. The laboratory provides a rich multi-disciplinary training environment and access to cutting-edge technologies in structural biology and drug development.

Further details of our most recent work can be found here:

  1. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y
  2. Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8

Preference will be given to applicants with demonstrated experience in protein purification from insect/mammalian cells and structural characterization of protein-nucleic acid complexes by X-ray crystallography or cryo-EM. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work independently but also effectively and collaboratively as part of a team. An ideal candidate should hold (or soon expect to hold) a Ph.D. or equivalent degree in a related discipline and have no more than 2 years of postdoc experience.

The Greehey Children’s Cancer Research Institute (GCCRI) is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. The GCCRI has significant external funding, a large endowment from the state, and links to philanthropic entities. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. There is no state income tax in Texas. All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities. Salary is competitive and commensurate with experience.

If interested in applying, please send a short description of your research accomplishments and a CV with contact information of 3 references to Dr. Yogesh Gupta (email: guptay@uthscsa.edu).


[Post Date: June 4, 2021]


Multiple positions for postdoctoral Fellow National Cancer Institute, Maryland

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Department of Health and Human Services (DHHS) National Institutes of Health (NIH) National Cancer Institute (NCI) Center for Cancer Research (CCR)

Five postdoctoral positions are available in The Molecular Basis of Disease Section (MBDS), Center for Structural Biology (CSB), Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Frederick, MD.  The candidate will perform basic research in molecular biology/structural biology/biochemistry.  

The MBDS group uses mixed structural methods to study host-pathogen interactions and other medically important molecular pathways with a particular focus on membrane resident protein complexes. We use and develop cutting-edge structural methods including cryo-electron microscopy and X-ray crystallography to define molecular mechanisms involved in health and disease states.

The research of the group is supported by the collaborative and interdisciplinary NIH intramural program, which includes more than 1100 labs and provides state-of-the-art equipment in structural biology, biochemistry, and biophysics. These core facilities are supported by Ph.D. level staff and offer hands-on training. In addition, NIH is committed to the continuing education and career development of its research staff through numerous courses and workshops offered by NIH Office of Intramural Training & Education (OITE) and Foundation for Advanced Education in the Sciences (FAES). There are also opportunities for intramural career transition funding (K grants).

Interested candidates must have a Ph.D. or M.D. degree with less than 5 years of postdoctoral experience.  Applicants must have a strong background in molecular biology, structural biology,  biochemistry, or a related discipline. The candidate should have good oral and written skills in English. The candidate should be skilled in at least one of the following areas: Biochemistry and molecular biology (experience in protein purification from different expression systems of soluble and membrane proteins); Electron microscopy (experience in cryo-EM sample preparation and digital image processing). We have excellent access to high-end microscopes (Titan Krios/Cold FEG/Selectris / Falcon IV, Arctica/BioQuantum/K3 and Aquilos II CryoFIB available locally in addition to smaller testing microscopes); Crystallography (experience in protein crystal structure determination). We have excellent access to Synchrotron beamlines and in-house X-ray diffraction and SAXS cores.

The candidate must be highly motivated to participate in rigorous and innovative research programs and possess excellent work ethic and team spirit, be able to work independently and efficiently and collaborate closely with members of the group, and other labs on joint projects.

The research group is supported by the collaborative and interdisciplinary NIH intramural and offers competitive salary and comprehensive health insurance.  Appointments will be one year initially and renewable up to a total of 3-5 years based on performance.  The NIH is dedicated to building a diverse community in its training and employment programs.  This position is subject to a background investigation.  

Interested applicants should send a cover letter indicating preferred starting date, a brief description (1-2 pages) of past research, CV, and contact information for at least three references via email to Susan Lea D.Phil.: LeaLabRecruit@nih.gov). More information about MBDS can be found at: https://ccr.cancer.gov/center-for-structural-biology/susan-m-lea. The Center for Structural Biology is located on the Frederick campus of NCI in Frederick, Maryland, USA. Frederick was founded in 1745 and is a city of ~70,000 inhabitants, 45 minutes from the central NIH, Bethesda Campus. Surrounded by State Parks, you can ski within 30 minutes of the campus in the winter and enjoy the many wineries, breweries and other thriving, independent, hostelries in the summer.

DHHS, NIH, and NCI are Equal Opportunity Employers

Recent Related Papers from Group:

Parker, J.L.^, Deme, J.C.^, Kuteyi, G., Wu, Z., Huo, J., Goldman, I.D., Owens, R., Biggin, P.C., Lea, S.M.* & Newstead, S.* (2021) “Structural basis for antifolate transport by the proton-coupled folate transporter PCFT” Nature https://doi.org/10.1038/s41586-021-03579-z

Johnson, S.^*, Furlong, E.J.^, Deme, J.C., Nord, A.L., Caesar, J.C., Chevance, F.V., Berry, R.M., Hughes, K.T. & Lea, S.M.* (2021) “Molecular structure of the intact bacterial flagellar basal body” Nature Microbiol., https://doi.org/10.1038/s41564-021-00895-y

Hennell James, R.^, Deme, J.C.^, Kjaer, A., Alcock, F., Silale, A., Lauber, F., Johnson, S., Berks, B.C.* & Lea, S.M.* (2021) “Structure and mechanism of a proton-driven motor that drives Type 9 secretion and gliding motility” Nature Microbiol. 6: 221-233.PMC

Deme, J.C., Johnson, S., Vickery, O., Muellbauer, A., Monkhouse, H., Griffiths, T., Hennell James, R., Berks, B.C., Coulton, J.W., Stansfeld, P.J. & Lea, S.M. (2020). “Structures of the stator complex that drives rotation of the bacterial flagellum.” Nature Microbiol. 5(12):1553-1564.PMC7610383

Kuhlen, L., Johnson, S., Zeitler, A., Baurle, S., Deme, J.C., Caesar, J.J.E., Debo, R., Fisher, J., Wagner, S. and Lea, S.M. (2020). "The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion." Nature Commun. 11(1): 1296.PMC7064499

Johnson, S., Fong, Y.H., Deme, J.C., Furlong, E.J., Kuhlen, L. and Lea, S.M. (2020). "Symmetry mismatch in the MS-ring of the bacterial flagellar rotor explains the structural coordination of secretion and rotation." Nature Microbiol. 5: 966-975.PMC7320910


[Post Date: June 2, 2021]


Structural Biologist (Protein Crystallography) SGC, Goethe University Frankfurt

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Description:

The Department of Biochemistry, Chemistry and Pharmacy of the Goethe University Frankfurt am Main offers immediately a position of a Postdoctoral scientist (m /f/ d)  (E13 TV-G-U) limited for initially 2 years. The salary grade is based on the job characteristics of the collective agreement applicable to Goethe University (TV-G-U). We are looking for a highly motivated and qualified biochemist with experience in protein purification and characterization as well as expertise protein crystallography for the development of specific inhibitors for novel E3 ligases („Structure-based discovery of small molecule inhibitors for novel E3 ligases“). We offer an exciting position embedded in an interdisciplinary, highly stimulating and international research environment at the life science campus Riedberg as well as ‘Landesticket Hessen’.

You have a master degree (or equal) as well as a PhD in biology, chemistry biochemistry, biotechnology or similar. You have profound knowledge in molecular biology and biochemistry and experience in protein crystallography. Practical experience in biophysical methods and experience of working abroad is a plus. You are comfortable to work within an interdisciplinary team of international scientists with different personalities and cultures. Fluency in English is a prerequisite. You are passionate about your work and have a proactive attitude.

We offer an exciting position embedded in an interdisciplinary research environment. Both positions are embedded in the Structural Genomics Consortium (SGC), a not-for-profit, public-private partnership encompassing 8 pharmaceutical companies that conducts pre-competitive research to facilitate the discovery of new medicines.  Based at several additional international universities (Stockholm, Toronto, Chapel Hill, Campinas) the SGC's work contributes to new hypotheses in understanding and treating human disease, and the subsequent identification of new targets for drug discovery.  The SGC is committed to Open Science, sharing knowledge, data and reagents, which has led to a collaborative network of more than 250 international partners. The project is part of the Target 2035 initiative, an international science federation with the goal to develop small molecule inhibitors for the human proteome. The first step is the IMI project EUbOPEN, an Innovative Medicines Initiative (IMI) funded project, which aims to generate specific chemical probes and assemble an open access chemogenomic library of well annotated compounds covering roughly 1,000 different proteins and test them in biological assays.

Goethe University is striving to increase the proportion of female researchers and encourages in particular women to apply for the position. People with disabilities will be given priority over other applicants with the same qualifications.

Interested candidates are encouraged to submit a cover letter stating career interests, CV and contact addresses of at least two referees in a single PDF until 15th June 2021 to the following e-mail address: Susanne.mueller-knap(at)bmls.de. Please note that application documents will be destroyed and not send back after the application procedure (do not hand in original documents) and travel costs cannot be refunded.


[Post Date: June 1, 2021]


Biochemist/Crystallographer at FoRx Therapeutics

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Description:

FoRx Therapeutics is a recently incorporated privately-held company based in Basel, Switzerland. It is backed by a syndicate of investors that include the Novartis Venture Fund, Pfizer Ventures, M Ventures, Omega Funds and LSP Venture Capital. FoRx Therapeutics focuses on drugging key molecular targets involved in the DNA Replication Stress, as a new approach in the development of targeted anticancer drugs.

We are seeking a Biochemist/Crystallographer to crystallize protein-drug complexes.

Requirements are a PhD in Biochemistry, Structural Biology or related Field. Postdoc experience is optional. First author publication in a relevant journal is required.

The successful candidate for this position will be a highly motivated individual who can communicate effectively, contribute beyond their strict discipline, be flexible and responsive to the changing needs of a dynamic entrepreneurial organization and fit well in small agile biotech team.

FoRx Therapeutics encourages and supports a diverse work environment as an Equal Opportunity Employer, offers a stimulating work environment and a competitive compensation package.

Interested candidates should send a cover letter describing research interests and career goals, along with a Curriculum Vitae to:

hr@forxtherapeutics.com 


[Post Date: June 1, 2021]


RNA Crystallography Senior Scientist

View the Job Posting Here

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Description:

Main Scope of Role:

  • Extensive knowledge in RNA biochemistry and macromolecular RNA crystallography (both RNA and RNA:Protein)
  • Experience with RNA handling
  • Familiar with RNA and protein design strategies
  • Familiar with Biophysics techniques (DSF, ITC…)
  • The successful candidate will have experience working in multi-disciplinary teams
  • Experience collaborating with scientists in external organisations

Key Responsibilities or Core Accountabilities:

  • Reading scientific literature relevant to the project
  • Supporting a project team by designing and ordering constructs to initiate the project
  • Adapting scientific knowledge to resolve problems encountered in achieving defined goals with assistance from line manager or other team members to meet internal and project deadlines
  • Preparing reports, presenting data, and collaborating with other scientists at Evotec
  • Presenting own work at project technical meetings, and department technical meetings when required
  • Demonstrating full command of all techniques and tools required for assigned responsibilities within the given project
  • Ensuring safe working practices of themselves and any direct reports
  • Complying fully with the company safety policy (including COPs and SOPs) when performing experiments
  • Adhering to Lab Notebook guidelines regarding accurate documentation of lab experiments

Knowledge, Skills & Capabilities:

  • Self-motivated, enthusiastic, cooperative and reliable
  • Knowledge of basic laboratory techniques, and understanding of the science underlying the techniques and experiments
  • Experience of working in a lab environment
  • The ability to adapt to changing demands whilst operating under pressure to deadlines
  • The ability to work accurately and with attention to detail
  • Good interpersonal skills and the ability to work well with other scientific staff
  • The ability to work well independently, and as part of a team
  • Good organisational and time management skills
  • Good verbal communication skills
  • Ability to maintain excellent laboratory records
  • Computer proficiency

Education, Qualifications and Experience:

  • PhD degree or equivalent in Structural Biology, Biochemistry or related area


[Post Date: May 28, 2021]


Two positions available at BioMAX (MAX IV)

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Description:

We are currently advertising two positions at the BioMAX beamline:  a beamline scientist (see https://lu.varbi.com/en/what:job/jobID:404484/) and a postdoc (https://lu.varbi.com/en/what:job/jobID:404444/ )

BioMAX  is the first macromolecular crystallography beamline at MAX IV. It  started user operation in 2017.  BioMAX  is a state-of-the-art beamline  with high performance x-ray optics and control systems.  It is geared towards high-throughput  protein crystallography and it can handle conventional MX experiments as  well as microfocus applications. For more information, see https://maxiv.lu.se/biomax.  BioMAX is operated by the international and interdisciplinary team, which is part of the MX group. The MX group is also  developing MicroMAX, a serial crystallography beamline for studying  protein dynamics and it manages a crystallographic fragment screening  facility,  FragMAX.

The successful candidates will be involved in the in-house research program and development of the beamline instrumentation, software  and methods to enhance the user facilities at BioMAX , with emphasis on  beamline automation, multi-crystal  experiments and room temperature data collection.

The application deadline is August 15th 2021

For  any inquiries about the positions, please contact Ana Gonzalez  (ana.gonzalez@maxiv.lu.se)  or Thomas Ursby (thomas.ursby@maxiv.lu.se)

  


[Post Date: May 28, 2021]


Sr. Research Associate, Structural Biology - Discovery Biology · San Diego, California

View the Job Posting Here

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Description:

Zentalis Pharmaceuticals is a San Diego based pharmaceutical company dedicated to the discovery and development of small molecule therapeutics targeting fundamental biological pathways of cancer. In the six years since our inception, we have successfully cleared four INDs with the FDA.  We believe our deep pipeline of oncology therapeutics has the potential to significantly improve the lives of patients with various types of cancer.

SUMMARY:

The Zentalis Structural Biology group is seeking an outstanding, detail-oriented, and motivated individual to join the scientific team. This individual will work both independently and collaboratively within the group as well as across multi-disciplinary drug discovery project teams. This position will be responsible for “gene to crystal” pursuits with particular emphasis on crystallization and small molecule co-crystallization of target proteins and protein complexes for X-ray crystallographic structure determination to enable structure-assisted optimization of lead compounds for oncology drug discovery programs.

RESPONSIBILITIES:

  • DNA expression construct design and generation for target protein expression; site-directed, insertional/deletional, and random mutagenesis/error-prone PCR
  • coli and insect cell recombinant protein expression leveraging different affinity tags and fusion proteins to enhance expression levels/solubility
  • Purification of protein from E. coli and insect cell hosts using FPLC systems by multiple affinity and separation techniques, affording mg quantities of crystallography-grade proteins for crystallization; proteolytic tag removal
  • Design, plan and execute crystallization trials using state-of-the-art commercially available sub-microliter crystallization instrumentation 
  • Crystallization of protein-small molecule complexes, identification of initial crystallization conditions and optimization, crystal soaking of small molecules, micro/macro seeding, crystal cryoprotection and harvesting in preparation for synchrotron data collection
  • Interpret crystal screening results, design and execute subsequent crystal optimization experiments from initial screening hits leading to well-ordered, diffraction-quality crystals affording high-resolution, quality structures
  • Peruse, read, and interpret primary literature involving structures of relevant and related oncology drug targets and apply this information to internal structure projects
  • Analyze, interpret and effectively report data at team meetings
  • Interface closely with structural biology colleagues to ensure robust purification schemes and crystal systems are established for targets in a timely fashion and aligned to the scientific project team goals
  • Work closely and collaboratively with structural biology colleagues to implement appropriate procedures, technologies, and instrumentation to establish and evolve a robust gene-to-structure workflow within the group
  • Maintain purification and crystallography instrumentation; ensure necessary crystallization reagents, supplies, equipment, and accessories are stocked
  • Other duties and projects as assigned

QUALIFICATIONS:

  • BS with 5-8 years, or MS with 3-5 years’ experience in an X-ray crystallography lab setting, preferably with an emphasis on early drug discovery and structure-based drug design, with degree in biochemistry, molecular biology, biology, or related field
  • Scientific expertise in gene to crystal processes, with extensive experience and demonstrated technical proficiency, problem-solving, and understanding of core concepts pertaining to cloning, protein expression/purification, and crystallization
  • Maintain comprehensive, up to date records of experimental methods, results, and observations with high level of accuracy; effective oral and written communication skills;
  • Knowledge of oncology drug discovery is preferred
  • Ability to multi-task/ prioritize and effectively manage time of daily activities
  • Intermediate to advanced software skills (e.g., Microsoft Excel, PowerPoint, MS Project, DNA/Protein Analysis software)
  • Flexibility within a rapidly changing environment
  • Proficiency functioning in a goal and team-oriented setting
  • Well-developed organizational skills

 Download PDF

[Post Date: May 27, 2021]


Crystallographer Exscientia

View the Job Posting Here

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Description:

Oxford, England / Biology / Full-time
 
Exscientia is at the forefront of Artificial Intelligence (AI) driven drug discovery.  Our AI driven systems actively learn best practice from vast repositories of drug discovery data and are further enhanced with knowledge acquired from seasoned drug hunters. Our team are passionate in the pursuit of excellence and at Exscientia you will enjoy a flexible, inspiring and dynamic working environment. Exscientia is an equal opportunity employer committed to building a culturally diverse team.
 
The Discovery Biology team in Exscientia is responsible for enabling small-molecule therapeutic projects by providing GPCR biology, protein production, biophysical and crystallographic capabilities. The team works on diverse target classes and strongly integrates with the Centaur Chemist AI platform to support the transformative efficiency improvements in clinical candidate discovery demonstrated with our systems.
 
We are looking to recruit an experienced scientist specialising in X-ray crystallography to support the discovery of new therapeutic molecules.  The candidate will be responsible for the crystallization and the structure determination of protein-small molecule complexes by x-ray crystallography. The work will include crystallization, X-ray data collection, structure determination, and analysis.

As the Crystallographer you will be responsible for

      • Performing high throughput crystallization experiments on multiple protein targets with and without ligands.
      • Optimize the initial crystallisation hits to obtain stable reproducible crystal systems.
      • Implementation of the established techniques of crystal manipulation including ligand soaking, crystal cryo-protection and harvesting.
      • Regular use of synchrotron facilities to execute standard x-ray data collection.
      • X-ray fragment screening experiments.
      • Attention to detail including good record keeping
      • To author high quality reports and data summaries.
      • To communicate updates to project meetings. 

      • Represent Discovery Biology at scientific meetings.
      • To work to the highest scientific standards under the direction of the Head of Crystallography.
 

What will you bring to the role?

      • Experience with high throughput crystallisation methods for screening using robotics.
      • Know-how of strategy design for crystal conditions optimization.
      • Skill in crystal manipulation, including soaking, cryoprotection, crystal harvesting.
      • Experience in synchrotron source X-ray diffraction experiments, data collection optimization and beamtime preparation.
      • Proficiency with X-ray data processing, structure solution and refinement. 
      • Knowledge of ligand chemical restraints definition and ligand structure refinement.
      • Excellent interpersonal intelligence
      • A demonstrable analytical approach to problem solving 
      • The ability to work as part of a team.  
 
Desirable: 
      • Understanding of all crystallographic methods (e.g. microbatch)
      • Comprehension of others structural biology techniques (e.g. SAXS and Electron Microscopy).
      • Experience in designing constructs for recombinant protein production of crystallographic targets. 
      • Previous work experience in an industrial environment.  
 
Qualifications: The successful candidate will possess a minimum of a strong honours degree plus either a postgraduate qualification up to PhD degree or proven industry laboratory experience in the life sciences. 

Reasons for joining us

    • Making a positive contribution to patients by revolutionising the pharmaceutical industry through AI-driven discovery. 
    • Kick-start or further grow your career - at Exscientia, you will have the opportunity to acquire valuable skills, work on problems that matter, and learn from world-class technical and scientific leaders.
    • Highly competitive compensation as we continue to grow and thrive.
    • State of the art laboratories and office space
    • Funding for professional development and conference attendance. 
    • Opportunity to join an inclusive, collaborative and intellectually stimulating culture
    • Health and well-being are important to us, we provide private healthcare, life assurance, wellbeing and mindfulness support.
    • Flexible working and remote working to allow you to manage your own time. 
    • A kitchen stocked with an endless supply of food for all employees
Exscientia embraces equal opportunity and is committed to providing a diverse and inclusive community where everybody feels welcome. We welcome applications from all people regardless of background. If you're looking for a new challenge within an exciting environment at the forefront of AI. then please apply today to learn more!


[Post Date: May 27, 2021]


Postdoc KU Leuven, Ligand-gated Ion Channels

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Description:

A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, KU Leuven, Belgium (http://www.xtal.be). The research in the Laboratory of Structural Neurobiology is aimed at understanding the molecular mechanism of ligand-gated ion channels. Our methods rely on X-ray crystallography and cryo-EM in combination with electrophysiological recordings and fluorescence-based techniques. Our laboratory hosts an integrated macromolecular crystallization facility with a Mosquito crystallization robot and two RockImagers. We have access to a 200 kV Glacios microscope on campus as well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current projects are situated at the crossroads of structural elucidation and drug discovery of different pentameric ligand-gated ion channels.

 We would like to recruit a post-doc with prior experience in cryo-EM or X-ray crystallography of membrane proteins.

The successful candidate:

  • easily communicates in an open verbal manner and has a team player spirit
  • has an ambitious work ethic
  • critically contributes to writing of manuscripts and grant proposals
  • makes efforts to obtain independent funding
  • meets intermediate deadlines and brings projects to a successful end
  • fuels internal discussions of recent publications in the field
  • sets an example to younger PhD students in the lab
  • identifies directions for future scientific research

The position is open immediately and applications will be accepted until the position is filled. Applicants are requested to submit a CV with publication list and two references. E-mail to chris.ulens@kuleuven.be


[Post Date: May 27, 2021]


Research Technician Baylor College of Medicine

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Description:

A Research Technician position is available in the Advanced Technology Core for Macromolecular X-ray Crystallography at Baylor College of Medicine in Houston, TX. The mission of the X-ray Core is to assist basic scientists and clinicians in finding structure solutions in order to develop novel treatments against unmet human diseases. The successful applicant will assist with the robotic crystallization screening and crystal optimization of proteins and protein-drug complexes. This is an entry level staff position that does not require prior experience with crystallization or structure analysis methods. Experience with protein expression and/or purification is an advantage. Salary is commensurate with experience and is in addition to a generous fringe benefit package.

Applicants must have a bachelor's degree in Biochemistry, Chemistry, or in one of the Natural Sciences, and must possess excellent verbal communication and writing skills. To apply, please send a current CV with relevant course work taken, a description of research experiences, and contact information for three referees to Dr. Sukyeong Lee, Associate Professor and Director of the X-ray Core Laboratory slee@bcm.edu.


[Post Date: May 20, 2021]


Position in Structural Studies of Ion Channels at the University of Chicago

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Description:

A full-time position for a postdoctoral researcher is available in the Department of Biochemistry and Molecular Biology at the University of Chicago to pursue structural studies on a novel ion channel using X-ray crystallography and cryo-electron microscopy, in combination with various biochemical, electrophysiological and computational techniques.

 

The ideal candidate will be an independent and highly motivated researcher with a strong background in membrane protein biochemistry and advanced techniques in protein crystallography.

 

Funding for the position is secured for at least 4 years, with opportunities for extension and, depending on performance, promotion to a "Staff Scientist" level position, allowing for considerable autonomy to develop independent research projects.

 

Please contact me directly if you are interested or know of a suitable interested candidate.  The search will continue until the position is filled.  Benoit Roux, Amgen Professor of Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, Department of Chemistry, Gordon Center of Integrative Science, University of Chicago.  roux@uchicago.edu


[Post Date: May 20, 2021]


Postdoce University of British Columbia, Vancouver

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Description:

A position is available for a highly motivated and skilled Post-doctoral Fellow (PDF) at the University of British Columbia in Vancouver, Canada.  The successful candidate will lead cryo-EM experiments to understand potassium ion channel complex gating and drug regulation in the heart. The position will be held within the Life Sciences Centre and Departments of APT and Biochemistry and Molecular Biology at the University of British Columbia (UBC), under the co-supervision of Dr. David Fedida and Dr. Filip Van Petegem.

The successful applicant must have a Ph.D. in structural biology. Excellent theoretical knowledge and practical skills in either X-ray crystallography or cryo-EM are required. Familiarity with the production and purification of membrane proteins is a major plus.  The position forms part of a collaborative, multidisciplinary effort between the Fedida and Van Petegem laboratories. As such, excellent oral and written English communication skills, the proven ability to work and as part of a team, and strong individual research productivity are essential.

Qualified Applicants should submit a cover letter including contact information for three professional references, and a curriculum vitae including a list of publications (n.b. do not include reprints). Application materials should be combined into a single PDF document and submitted via email to David.fedida@ubc.ca and filip.vanpetegem@gmail.com

The position is available immediately. The appointment will be at the rank of Postdoctoral Fellow with a salary commensurate with research experience, plus benefits (see https://hr.ubc.ca/benefits/eligibility-enrolment/eligibility-and-plan-cost/faculty-postdoctoral-fellows-employees-and ).

Equity and diversity are essential to academic excellence. An open and diverse community fosters the inclusion of voices that have been underrepresented or discouraged. We encourage applications from members of groups that have been marginalized on any grounds enumerated under the B.C. Human Rights Code, including sex, sexual orientation, gender identity or expression, racialization, disability, political belief, religion, marital or family status, age, and/or status as a First Nation, Metis, Inuit, or Indigenous person.


[Post Date: May 19, 2021]


Structural Biology Faculty Position, UConn Health Center (UCHC), Assistant/Associate Professor

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Description:

UCONN Health

ASSISTANT/ASSOCIATE PROFESSOR - BASIC SCIENCES

The Department of Molecular Biology and Biophysics (MBB) at UConn Health, Farmington, CT, seeks exceptionally creative candidates for an early-to-mid career position (tenure-track Assistant or Associate Professor), who use molecular and translational approaches to elucidate the molecular basis of disease.

Research in MBB integrates multiple approaches to molecularly define the proteins and pathways affected in human disease. Our studies span the structural biology of cancer drug targets to the mechanistic interplay between viruses and human cells. We seek creative and innovative candidates whose research interests and accomplishments complement and further existing strengths in the department and the larger UConn Health research community. Ideal candidates will have a research program focused on molecular signaling (including but not limited to areas such as posttranslational modifications, DNA repair, protein ubiquitination, kinase/phosphatase signaling/regulation) and/or the structure, function and regulation of proteins involved in signaling processes. Programs that focus on the identification and development of translational outcomes, including probes that will allow for in-depth analysis of key biological pathways or novel drug-like molecules, are also of particular interest.

MBB and UConn Health provides a rich and diverse research environment and is deeply committed to the development and success of its incoming faculty. The UConn Health School of Medicine is home to 30 departments and institutes, including Cell Biology, Genetics and Genome Sciences, the Richard D. Berlin Center for Cell Analysis and Modeling, among others. It is also closely affiliated with The Jackson Laboratory, which is housed on the same campus. Candidates will also have access to 22 exceptional core facilities, including the CCAM Microscopy, NMR Structural Biology and Biophysics, High-Performance Computing, EM, MS, FACS and Histology core facilities. UConn Health also offers competitive salaries and excellent benefits.

Successful applications will have a PhD, MD or equivalent degree; a sustained record of exceptional scholarly success; and the promise of future innovative accomplishments via a proposed (early-career) or established (mid-career, including evidence of successful extramural grant writing) research programs that utilizes state-of-the-art molecular, genetic, cellular, biochemical, biophysical and/or structural methodologies. Candidates are also expected to be highly collaborative and integrate well with the growing number of researchers and physician scientists in the basic and clinical departments of UConn Health. Candidates with ongoing funded research are encouraged to apply.

Please visit https://jobs.uchc.edu/CSS_External/CSSPage_Welcome.asp and select 'Molecular Biology and Biophysics' under 'department' (job number 2021-650). 

To apply, interested individuals should submit a curriculum vitae, cover letter, statements of research plans (up to 3 pages) and teaching (up to 1 page) and arrange for the submission of three letters of reference on letterhead with signature.

Questions regarding this position should be addressed to Wolfgang Peti, Department Head, Molecular Biology & Biophysics at peti@uchc.edu.

UConn Health is an affirmative action employer, in addition to an EEO and M/F/V/PWD/PV employer.


[Post Date: May 19, 2021]


Multiple Job Openings at Peak Proteins

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Description:

There are currently a number of job openings at Peak Proteins Ltd. for exceptional protein scientists including graduate level positions right up to Director.

If interested more information including job descriptions can be found here: https://peakproteins.com/join-us/

Peak Proteins is a vibrant contract research organisation (CRO) that provides research services to clients globally and is located on the Alderley Park site in Cheshire; only 18 miles from the heart of Manchester and has easy access to the Peak District, Snowdonia and Lake District national parks.


[Post Date: May 19, 2021]


Research Associate Protein Purification/X-ray Crystallography New York Structural Biology Center

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Description:

Job Description:

Full Time Research Associate (protein purification/crystallization)

The New York Structural Biology Center is a leading research institution located in Manhattan specializing in structural biology of macromolecules. We are seeking a Research Associate to work in our protein production/X-ray crystallography group. Duties will focus on cloning of expression constructs, protein expression/purification, and crystallization experiments.

We seek a candidate with a master's degree in biology, biochemistry, or a closely related discipline, who will be working on a team with other research associates under guidance and direction from PhD level scientists. Bachelor's degree candidates may be considered if the candidate can show extensive practical experience in the field. We will NOT consider any PhD level applicants for this position.

Responsibilities include, but are not limited to, the following:

- cloning of expression constructs

- protein expression and purification from bacterial systems

- downstream protein biochemistry and protein crystallization experiments

- general laboratory maintenance and ordering of supplies

The successful candidate will be responsible for cloning expression constructs, protein expression and protein purification using GE Healthcare FPLC/AKTA chromatography systems, and protein crystallization trials. It is crucial that the candidate be fluent in working with computer-controlled equipment and computer interfaces.

Candidates must meet the following requirements to be considered:

- a master's degree in biology, biochemistry, or a closely related discipline

- extensive experience with bacterial protein expression

- experience with GE Healthcare FPLC/AKTA protein purification systems

- fluent in working with computer-controlled equipment and computer interfaces

- proven ability to multi-task on multiple independent projects at the same time

- excellent communication and organizational skills

- ability to work in a team environment with others

Salary level is commensurate with qualifications and experience. Please send a copy of your resume to nysbcjobs@gmail.com in your reply.


[Post Date: May 18, 2021]


Postdoctoral Position in Structural Biology

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Description:

Location: Cambridge MA

Cambridge, MA-based H3 Biomedicine (H3B) is a leading company in cancer genomics driven drug discovery, delivering on the promise of precision medicine. The company was launched in 2011 with an unprecedented $200 million in initial funding from Eisai, plus a continued ongoing commitment to fund H3B’s robust discovery pipeline and clinical programs. Now with three clinical stage anti-cancer molecules in active development, H3B is able to uniquely leverage distinct insights from cancer genomics and real-life patient data to advance its projects to clinical proof of concept and beyond. H3B embraces a novel business model in which it collaborates with Eisai, a leading global pharmaceutical company, to create a prolific drug discovery engine and partnership platform.

We have an exciting opportunity to join a fantastic team of scientists as a postdoc in structural biology.  H3 Biomedicine is at the forefront of cancer genomics and drug discovery.  Structural biology plays a prominent role in underpinning our mechanistic understanding of disease biology and empowering our drug design.

Principal Duties and Responsibilities
  • Function as an outstanding protein chemist with a PhD in structural biology with an emphasis in cryo-EM or x-ray crystallography.
  • Assist with a range of projects in our oncology portfolio from early to late stage with numerous opportunities for publication.
    • These projects include elucidating the structures of large proteins (>150 KD) and protein complexes that will provide new insights into cancer biology.
    • To enable these projects, H3 has invested in a GPU workstation for in house data processing / reconstruction and has entered into relationships with both U Mass and Nanoimaging for data collection.
  • The successful candidate will also be encouraged to collaborate and consult with academic advisors as needed.
  • We desire an individual with attention to detail, motivation, independence, and ambition to further our mission to improve patient lives.
Qualifications
  • Construct design
  • Expression systems (E coli, insect cells, and mammalian)
  • Experienced AKTA user
  • Western Blots
  • Cross-linking techniques
  • Sample prep for Negative stain and Cryo-EM techniques
  • Data processing (particle picking, 2-D classification, 3-D reconstruction, Model Building, Refinement)
  • Software CCP4, CCP-EM, RELION, cisTEM, Coot, Pymol
  • Excellent written and verbal communication
  • Nice to have: crystallization techniques (basics screening, optimization, seeding, soaking, harvesting, freezing)
About H3 Biomedicine Inc.

H3 Biomedicine Inc. is a privately-held, uniquely-structured oncology discovery enterprise whose sole mission is to become a prolific source of new drugs that treat more human cancers with greater success. H3 Biomedicine is applying the expertise of leading scientists to the integration of insights from cancer genomics with innovative capabilities in synthetic chemistry and tumor biology to pursue the most promising current opportunity in cancer therapeutics: patient-based, genomics-driven, small molecule drugs.


[Post Date: May 18, 2021]


Postdoc Structural Biology University of Texas Health Science Center at San Antonio

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Description:

A postdoctoral position is immediately available for a highly motivated individual interested in understanding molecular mechanisms of ubiquitin signaling.  Research is focused on the enzymatic machinery responsible for protein ubiquitination, characterization of PROTAC complexes with potential therapeutic value, and structure determination of large protein complexes involved in ubiquitin signaling, particularly those regulating DNA repair.  These issues will be addressed using a multidisciplinary approach that includes cryo-EM, X-ray crystallography, and NMR complemented with biochemical/biophysical techniques and cell-based assays. 

Candidates should hold a recently obtained PhD in biochemistry, chemistry, or related subject, with demonstrated expertise in cryo-EM, X-ray crystallography, and/or the biochemical analysis of protein ubiquitination.  Experience with protein expression in insect and mammalian cells is a plus.

Interested candidates should e-mail a cover letter, CV, and the names of three references to: olsens@uthscsa.edu.


[Post Date: May 17, 2021]


Postdoc Structural Biology St. Jude Children's Research Hospital

View the Job Posting Here

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Description:

Summary

STRUCTURAL BIOLOGY postdoc project

Small Molecule Degraders for Targeting Transcription Factor Drivers in Childhood Cancers

 

We are recruiting a postdoc for a multidisciplinary, collaborative project that exploits targeted protein degradation (TPD) of pediatric cancer targets using Molecular Glues (MG). TPD is an exciting and rapidly evolving drug discovery paradigm that shows great promise for drugging currently undruggable targets. In this recently funded 5-year project we are developing novel MG approaches to target key transcription factor dependencies in high-risk childhood acute leukemia and medulloblastoma.

 

We are looking for highly motivated, brilliant minds with curiosity and skills to contribute structural biology insights that shed light on MG interactions with ternary complexes of neosubstrates such as oncogenic transcription factors. Such insights will aid target characterization, MG hit optimization, structure-guided molecular design, all the way to preclinical validation and subsequent clinical translation. These structural biology efforts are integrated into a highly collaborative environment comprised of chemists and pathologists, as well as computational biologists, systems biologists and experimental in vitro/ in vivo biologists that work closely together toward the common goal of improving childhood cancer outcomes.

 

The project is housed in the Department of Chemical Biology and Therapeutics, and Structural Biology at St. Jude Children’s Research Hospital. It builds on strong investments into a world-class infrastructure in structural and chemical biology. This is an exciting time to join us and build a career while living in an affordable city. This postdoc position is funded for 5 years.

 

Please direct your questions and application package including a cover letter, current CV, and 3 letters of reference to: Dr. Marcus Fischer (marcus.fischer@stjude.org).

 

https://www.stjude.org/fischer

 

https://www.stjude.org/media-resources/news-releases/2021-medicine-science-news/st-jude-receives-9-9-million-to-advance-cures-for-hard-to-treat-pediatric-cancer.html

Minimum Education

  • PhD in structural biology

Minimum Experience

Essential requirements:

  • Independence in gene-to-structure workflow
    • Demonstrated proficiency in molecular biology, construct design, cloning, protein biochemistry, expression (including insect cells), purification, reconstitution and structural determination of ternary complexes
  • Strong background in X-ray crystallography
    • automation, optimization, crystallization robotics; synchrotron data collection
    • structural analyses, representation and integration with other biophysical data 

Optional expertise:

  • Experience in cryoEM, xFEL or microED
  • Experience with computational approaches for automation and data analysis and in silico modelling

EEO

St. Jude is an Equal Opportunity Employer

No Search Firms:

St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.


[Post Date: May 17, 2021]


Crystallography Senior Scientist

View the Job Posting Here

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Description:

Required knowledge, skills and abilities:

  • Extensive knowledge in protein biochemistry and macromolecular crystallography
  • Experience with protein purification
  • Protein engineering, bioinformatics and molecular biology (Construct design and cloning strategy)
  • Familiar with Biophysic techniques (DSF, ITC…)
  • A working knowledge and practical experience with either E. coli, insect or mammalian expression systems
  • The successful candidate will have experience working in multi-disciplinary teams
  • Experience collaborating with scientists in external organisations

Responsibilities includes:

  • Reading scientific literature relevant to the project
  • Supporting a project team by designing and ordering constructs to initiate the project
  • Adapting scientific knowledge to resolve problems encountered in achieving defined goals with assistance from line manager or other team members to meet internal and project deadlines
  • Preparing reports, presenting data, and collaborating with other scientists at Evotec
  • Presenting own work at project technical meetings, and department technical meetings when required
  • Demonstrating full command of all techniques and tools required for assigned responsibilities within the given project
  • Ensuring safe working practices of themselves and any direct reports
  • Complying fully with the company safety policy (including COPs and SOPs) when performing experiments
  • Adhering to Lab Notebook guidelines regarding accurate documentation of lab experiments

Desirable Knowledge, Skills and Abilities:

  • Experience in Cryo-EM
  • Self-motivated, enthusiastic, cooperative and reliable
  • Knowledge of basic laboratory techniques, and understanding of the science underlying the techniques and experiments
  • Experience of working in a lab environment
  • The ability to adapt to changing demands whilst operating under pressure to deadlines
  • The ability to work accurately and with attention to detail
  • Good interpersonal skills and the ability to work well with other scientific staff
  • The ability to work well independently, and as part of a team
  • Good organisational and time management skills
  • Good verbal communication skills
  • Ability to maintain excellent laboratory records
  • Computer proficiency

Education & Experience:

  • PhD degree in Structural Biology, Biochemistry or related area


[Post Date: May 17, 2021]


Postdoc University of Kentucky, USA

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Description:

A postdoctoral position is available immediately in the laboratory of Dr. Emilia Galperin in the Department of Molecular and Cellular Biochemistry in the College of Medicine at the University of Kentucky to work on externally funded, multi-year projects. The overall goal of the lab is to understand the molecular mechanisms of spatial and temporal regulation of signal transduction. Preference will be given to applicant with a strong background in protein purification and characterization; experience in other biophysical methods such as x-ray crystallography is a plus. The successful applicant will be a highly motivated individual with good communication skills, will have a proven publication record, and the ability to work independently as well as within a group. This work is in collaboration with the protein structural group of Dr. David Rodgers.

The applicant must currently have a Ph.D. in Biochemistry or a related field. The appointment will be for 1 year with renewal up to 5 years. Interested candidates should assemble a i) cover letter, ii) CV, iii) list of references, and iv) first-author manuscripts that have been published or accepted for publication. The cover letter should describe the candidate's research experience, scientific interests, and preferred start date.

E-mail the application as a single PDF file to emilia.galperin@uky.edu.


[Post Date: May 16, 2021]


Postdoc Structural and Functional Characterization of Macromolecular Complexes in Transcription and Repair

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Description:

A postdoc position, supported by NIH funding, is available in the Dong Wang Lab at University of California, San Diego.

Our research focuses on the interplay between two fundamental processes: transcription and DNA repair. Current research projects focus on the X-ray and CryoEM structural elucidation and functional/biochemical study of macromolecular protein-nucleic acid complexes involved in transcription, chromatin remodeling, and DNA repair. Please visit https://dongwang.ucsd.edu for details of our recent research and publications.

We are looking for a highly motivated individual with experiences in biochemistry, and molecular biology, X-ray crystallography, or CryoEM. We sincerely invite you join our small, but vibrant and highly productive team. Candidates must have at least one original first author publication in the related field in an internationally recognized journal. Please send your CV, list of publications, and the names and Email addresses of 2-3 references to dongwang@ucsd.edu before Oct 15, 2021.


[Post Date: May 13, 2021]


Postdoctoral Research Associate in Structural and Molecular Biology in Metropolitan Chicago – The Chicago Medical School, Rosalind Franklin University

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Description:

Position Summary

An NIH Funded position for studies on the mitochondrial ATP synthase and on Batten disease, a childhood neurodegenerative disease. This is an exciting opportunity to gain experience in many facets of protein expression, purification, and crystallization, nanodiscs preparation, yeast fermentation, and more. Experience in biochemistry and molecular biology is essential. The candidate must be willing to learn new skills, interact with others, keep a detailed notebook, and eventually, to work independently. We are goal oriented – reasonable goals are set for each day and week – and thus the hours in each workday or week can vary. 

The position will be titled Research Assistant, Research Associate, or Postdoctoral Fellow depending on the education and experience of the selected candidate. The salary is commensurate with experience.  Candidates identified as a Postdoctoral Research Associates/Fellows will be paid according to NIH/NRSA standards for postdoc compensation. The university offers generous health care, retirement, paid vacation and sick day benefits.

Essential Duties & Responsibilities  

  • Construct gene constructs using standard Molecular Biology methods. 
  • DNA sequence analysis and interpretation. 
  • PCR, subcloning, and DNA plasmid preparation. 
  • Construction of deletion mutations in yeast genome. 
  • Growth of yeast in a 60l fermenter. 
  • Protein purification and assay. 
  • Preparation of nanodiscs for cryo-EM 
  • Set crystal screens and test crystals for X-ray diffraction. 
  • Aid in x-ray data collection.
  • Learn additional skills as needed. 
  • Perform other related duties as needed.

Additional Responsibilities for candidate at the PhD/Postdoctoral Level

  • Assist in writing manuscripts
  • Read current literature
  • Give Journal club papers
  • Train new members as needed.

Minimum Required Education & Experience

  • B.S. in Biochemistry, Chemistry or related field
  • A PhD received within the last 6 years is required to be considered for a Postdoctoral Research Associate / Fellow
  • Must achieve satisfactory results from a background check

Required Knowledge, Skills, & Abilities 

  • Fluent in English. 
  • Excellent reading, writing and communication skills. 
  • Technical requirements include skills in Biochemistry and Molecular Biology.
  • Ability to work well within a group setting. 
  • Highly independent and self-motivated. 
  • Familiar with standard Windows Office software, spreadsheet applications, and graphics applications. 
  • Ability to work and interact well with others of diverse backgrounds. 
  • Extreme attention to detail with good notebook keeping
  • Flexibility in working hours (long days and weekends if needed). 
  • Ability to collect and analyze data.

Additional Required Knowledge, Skills, & Abilities at the PhD/Postdoctoral Level 

  • Must demonstrate strong leadership experience. 
  • Demonstrated project management skills, including ability to set priorities and excellent time management skills.
  • Excellent organizational and planning skills. 
  • Ability to collect and analyze complex data. 

Preferred Qualifications

  • Skills in X-ray crystallography or cryo-EM.  


[Post Date: May 3, 2021]


Protein Scientist Broad Institute MIT

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Description:

The Zhang lab (Broad Institute and MIT) is seeking to recruit a full-time staff research scientist to contribute to our on-going efforts to biochemically characterize novel natural systems and engineer them to develop biotechnologies that improve our planet and people’s lives.

The Zhang lab has pioneered a number of new biotechnologies, including CRISPR-mediated genome editing, new approaches for high-throughput genetic screening, DNA microscopy, CRISPR-based diagnostics, and single-cell genomics methods. These advancements often stem from natural systems, and the lab is also at the forefront of discovering and characterizing novel molecular systems. Collectively, our work encompasses many disciplines, including computational biology, evolutionary biology, microbiology, biochemistry and structural biology, molecular biology, genomics, and bioengineering. Our lab is a fun, vibrant, fast-paced, results-driven, nurturing, and collaborative environment.

We are looking for a talented and skillful scientist to help experimentally characterize novel biological systems and determine their function, with an eye toward harnessing them for molecular biotechnologies. Experience with protein purification, structural biology, biochemical assays, or protein engineering is required, and familiarity with protein-nucleic acid complexes is desirable. The candidate will collaborate with existing lab members or new hires as needed to advance the project.

 

The Broad Institute of MIT and Harvard offers an outstanding place to work. The workplace culture is diverse, dynamic, progressive, and supports a healthy work-life balance. To support this mission, the Zhang lab strives for a workplace culture that supports diversity, intellectual curiosity, flexibility, and integrity. Broad employees receive a generous benefits package (including a salary competitive with industry, dental, vision and health insurance, retirement plan, and additional benefits) and a range of possibilities for career advancement. Details on our benefits package can be found here - https://www.broadinstitute.org/benefits/benefits-working-broad

PRINCIPAL DUTIES AND RESPONSIBILITIES:

  • Purify proteins and protein-nucleic acid complexes
  • Perform biochemical assays to characterize proteins and their nucleic acid components
  • Stay current on the field and think deeply about creative new ways to have an impact
  • Contribute to the lab through teamwork and discussion
  • Help to train, supervise, and mentor junior staff as needed

MINIMUM QUALIFICATIONS:

  • MSc or PhD in a relevant field (e.g., biochemistry, molecular biology).
  • Eager to work in a team and collaborate
  • Demonstrated scientific contributions to the field
  • Superior experimental planning skills
  • Proven ability to efficiently and effectively solve problems
  • Outstanding clarity of thought in communications (written and oral)

Please send CV to zhangrecruiting@broadinstitute.org if interested.


[Post Date: April 30, 2021]


Postdoc & Technician Positions at Brown University, USA

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Description:

The Deaconescu Lab at Brown University has one opening for a postdoctoral researcher and one technician. The laboratory's interests lie primarily in stress responses, with particular emphasis on responses to DNA damage and the mechanochemistry of DNA-based motors. We utilize a combination of biochemical, biophysical and structural techniques. (e.g. X-ray crystallography, electron microscopy). Examples of recent work are: Schwartz et al, Protein Science (2021), Brugger et al, Nature Communications (2020), Dorich et al, Genes & Development (2019), Vemu et al. Science (2018), Le TT et al. Cell (2018), Szyk et al, Cell (2014).

A successful postdoctoral candidate should have an established track-record of publications in peer-reviewed journals; solid experience with protein biochemistry (including their purification, functional characterization and assay development). We have a variety of projects available at different stages of development, including some that involve drug design. Prior knowledge of crystallography and/or single-particle electron microscopy is highly desirable, but not required. Working knowledge of bacterial genetics could also be an asset.  Must be highly motivated and work well independently as well as in a team. Excellent spoken and written English are required. New Ph.D. graduates are encouraged to apply. 

A successful technician candidate should preferably have  a degree in chemistry/biological sciences and more than two years experience at the bench, at the minimum with molecular biology, and ideally, also with protein purification and crystallization.

Interested candidates should send a CV, a one page research experience summary and contact information for three references to alexandra_deaconescu[at]brown.edu. Please use “postdoctoral candidate” or “technician candidate" as a subject.

Salary and starting date are negotiable.   Please write Postdoctoral Candidate in the e-mail subject header.   Brown University, an Ivy League school ranked as 5th best in the US by the Wall Street Journal, is located in Providence, Rhode Island less than one hour away by train from Boston.   Rhode Island provides plenty of opportunities for outdoor recreation with beautiful beaches and sailing.


[Post Date: April 30, 2021]


Postdoc Opportunity at Harvard Medical School

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Description:

A postdoctoral position is available immediately in the laboratory of James Chou at Harvard Medical School. 

The research project investigates the structural and physical basis of immune receptor autoinhibition and activation. The goal is to use the knowledge to guide the development of true agonistic therapeutics for cancer immunotherapy. For general information of our lab, please visit https://chou.hms.harvard.edu/.

The applicant must have a PhD and solid training in protein biochemistry, structural biology, or cell biology. Interested applicants should directly contact James Chou at james_chou@hms.harvard.edu.


[Post Date: April 27, 2021]


Postdoc Page Laboratory University of Connecticut Health Center

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Description:

The Page Laboratory at the University of Connecticut Health Center has an immediate opening for up to two post-doctoral candidates to study pro- and eukaryotic signaling enzymes, with a focus on the function of phosphatases that form large signaling complexes, and also drug/inhibitor development. All studies use X-ray crystallography and/or cryo-EM/NMR. Applicants with a strong background in biochemical techniques, and especially eukaryotic protein expression and protein crystallization, are encouraged to apply.

The Page laboratory is housed in a newly renovated ~250,000 sq. ft. research laboratory in Farmington, CT. The laboratories are open-lab-space facilities and allow close interaction with the adjunct structural biology and molecular/cell biology groups at UConn Health. The Page laboratory is equipped with all necessary infrastructure to produce (E. coli, Sf9, mammalian), purify and analyze protein and protein complexes (ITC, SPR etc.) and crystallization robotics for crystal production.

Farmington, CT, is located on the New England between Boston (90 min.) and New York City (90 min.). Starting date, salary and project details are negotiable.

Please e-mail a Cover Letter and CV to Rebecca Page (rpage(at)uchc.edu).

Recent publications:

  • Wang, X., Garvanska, DH, Nasa, I, Ueki, Y, Zhang G, Kettenbach, AN, Peti, W, Nilsson, J, Page, R (2020). A dynamic charge-charge interaction modulates PP2A:B56 substrate recruitment. eLife. 9. Pii: e55966.
  • Choy, M.S., Moon, T.M., Ravindran, R., Bray, J.A., Robinson, L.C., Archuleta, T.L., Shi, W., Peti, W., Tatchell, K., Page, R. (2019) SDS22 selectively recognizes and traps metal-deficient inactive PP1. PNAS 116:  20472-20481.


[Post Date: April 27, 2021]


Postdoc National Cancer Institute, NIH in Bethesda, Maryland, USA

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Description:

The Section of Structural Biology of Membrane Proteins in the Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH has an opening for Postdoctoral Fellow with a recent Ph.D. in Biology and training in cryo-EM and/or X-ray crystallography. This individual will be working on "Mechanistic investigation of cellular drug resistance ".  The project involves structural studies of cytosolic and mitochondrial proteins that are targets of antimicrobial compounds.  It is anticipated that multiple structural techniques will be employed, involving the use of X-ray crystallography and Cryo-EM to illuminate structural aspects of target protein’s response to drug treatment.  Our group has assured access to a synchrotron beam line at APS and to an intramural EM facility equipped with an FEI Krios microscope and Gatan K3 detector. The successful candidate should have practical experiences in molecular biology and protein purification for target protein production. Experience in mitochondrial proteins is a plus. NIH has a welcoming, collaborative and vibrant environment for biomedical research. Our campus is located in Bethesda, Maryland. Interested individuals should send their Applications, consisting of a CV, a statement of research interest, and three letters of recommendation, to Dr. Di Xia via Email at xiad@mail.nih.gov


[Post Date: April 27, 2021]


Postdoc Albert Einstein College of Medicine

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Description:

The Almo lab in the Department of Biochemistry at the Albert Einstein College of Medicine seeks applicants for a post-doctoral fellow to work on the structural and functional characterization of cell surface and secreted molecules for the development of a broad range of immunotherapeutic applications. The successful candidate will have extensive experience in eukaryotic expression platforms as well as oxidative refolding of bacterial inclusion bodies. Familiarity with covalent coupling chemistry and/or enzyme-mediated coupling approaches is desirable. This program seeks to develop new strategies and biologics to study and treat malignancies, autoimmune diseases and infectious diseases. The Almo lab offers an outstanding working environment with state-of-the-art infrastructure in high-throughput eukaryotic expression, functional annotation and structure determination, while the College is home to internationally acclaimed experts in modern immunobiology.

Applicants should have a Ph.D. or equivalent degree. Interested candidates should contact Steve Almo (steve.almo<at>einsteinmed.org). Please provide a CV, including the names of three references.

The Albert Einstein College of Medicine is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information. Einstein seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve.


[Post Date: April 27, 2021]


Postdoc Time-Resolved Serial Synchrotron Crystallography at ESRF ID29

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Description:

Context & Job description

As part of the Extremely Brilliant Source facility upgrade (https://www.esrf.eu/about/upgrade), the ESRF is constructing a new beamline for serial and time-resolved macromolecular crystallography (TR-)SSX experiments at room temperature. The new ID29 beamline will deliver an unprecedented flux density, allowing the recording of diffraction data from micron-sized samples in a few microseconds, and will be equipped with a next generation diffractometer and a Jungfrau detector. Beamline operations will be supported by a dedicated sample preparation laboratory where the most suitable crystal delivery systems such as injectors, fixed targets and microfluidic devices will be prepared and characterised prior to experiments.

The postdoctoral position available will focus on developing novel sample environments, delivery systems and data collection methods for (TR-SSX). These will, initially, be tested and commissioned at other ESRF MX microfocus beamlines. The selected candidate will also take part in the commissioning of the new ID29, performing state-of-the art experiments at the new end-station.

As for all post-doctoral researchers in the Structural Biology Group, the successful candidate will participate in in-house research programmes, will contribute to the operation of the beamline and will provide support to external users as local contact. He/She will also have the opportunity to develop his/her own research programme.

Expected profile

  • PhD in chemistry, physics, biophysics, biology or closely related science obtained less than 3 years ago
  • A strong background in X-ray crystallography and serial crystallography is desirable
  • A strong interest in new method development
  • Ability to interact with multi-disciplinary staff and facility users
  • Proficiency in English (working language at the ESRF)

Working conditions

Contract of 18 months, renewable for a further 6 to 18-month period.

We offer a comprehensive benefits package designed to support your quality of life and your installation in the Grenoble area when relocation is needed.

The annual gross salary may be completed by additional allowances (shift allowance, standby allowance, expatriation allowance, etc.).


[Post Date: April 22, 2021]


Structural Biology and Biophysics Research Scientist

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Description:

Job Description

We are currently seeking a self-motivated scientist with experience in the field of Protein Production, Structural Biology and Biophysics. The successful candidate will be joining a team of biophysicists and structural biologists focused on delivery of transformative medicines for a range of serious genetic diseases and conditions with an unmet medical need. You will be working closely with teams across Vertex in our early stage portfolio.  This position provides the candidate with a world-leading opportunity to learn and develop skills in drug discovery in a patient-focused environment.

The successful candidate will be able to demonstrate relevant academic or industrial experience with challenging targets such as protein complexes and/or membrane proteins. An ability to critically assess the scientific literature, design and run your own experiments is a key feature for this role. An innovative and “can-do” attitude is essential for working in our high throughput and high impact team.

You will have strong hands-on experience in mammalian protein expression in mammalian or Baculovirus expression systems and purification of challenging targets. Practical expertise in solving and interpreting structures of macromolecules using techniques such as cryo-EM and X-ray crystallography with particular application to membrane proteins and/or large protein complexes will be an essential component of this role. Hands-on experience in a broad range of biophysical or biochemical techniques, preferably using high throughput methods, would also be an advantage.   

You will be part of a growing, high impact team exploring and implementing new ideas in protein production, structural biology and biophysics to increase efficiencies and drive innovation. You will be working collaboratively with multiple interdisciplinary teams both on site here in the U.K. and globally, to understand and fulfil the demands of the specific projects by establishing efficient work plans and developing new protocols.

The successful candidate will have an exciting opportunity to contribute to high impact and strategic science across our early research portfolio. As a key player within multi-disciplinary drug discovery teams, you will be exposed to every aspect of drug discovery and development in one of the world's leading biotechnology companies with an uncompromising commitment to patients.

Key responsibilities

  • Maintaining an in depth understanding of the biochemistry, molecular structure and wider role of each target in disease through critical analysis of the literature, working closely with our disease biology teams.
  • Purification of proteins and other macromolecules for structural, biophysics and high throughput assays
  • Determination of structure of macromolecules by X-ray crystallography, cryo-EM and/or cryo-ET.
  • Biophysical and biochemical studies of macromolecules using a range of methods for target engagement and small molecule modulator development.

Key requirements

  • Ph.D. level Biochemist or Biologist with extensive proven laboratory experience from similar roles in the pharmaceutical industry setting and/or in academia.
  • A track record in delivering high impact results in biophysics, structural biology and protein science within academic or pharmaceutical industry settings as demonstrated through a strong publication history, supported by evidence of individual contributions to projects.
  • Practical experience of recombinant protein expression in several mature platforms including mammalian (stable and transients), insect cells and E.coli. Strong practical experience in protein purification including IMAC, IEC, SEC, IEX, HIC, fusion protein cleavage technologies, use of AKTA system-based chromatography.
  • Practical experience in structural biology, using X-ray crystallography and/or electron cryo-microscopy with a focus on membrane proteins and/or protein complexes.
  • Experience in the use of a range of biophysical methods, particularly in the context of assessing small molecule target engagement.
  • Familiarity with high-throughput methods for assessing protein expression and purification yields and quality.
  • Strong understanding of molecular biology, protein engineering, mass spectrometry, assay development, electrophysiology and/or high-throughput screening workflows would be an advantage.
  • Strong interpersonal and troubleshooting skills.
  • Demonstrate excellent record keeping, communication and presentation skills.
  • Demonstrable leadership for promoting innovation in those techniques and demonstrable abilities to troubleshoot problems as they arise.

Company Information

Vertex is a global biotechnology company that invests in scientific innovation.

Vertex is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry, ethnicity, disability, veteran status, genetic information, sexual orientation, marital status, or any characteristic protected under applicable law. Vertex is an E-Verify Employer in the United States. Vertex will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law. Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the recruiter or hiring manager, or contact Talent Acquisition at ApplicationAssistance@vrtx.com.


[Post Date: April 22, 2021]


Biochemist/Assay Development Scientist at FoRx Therapeutics

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Description:

FoRx Therapeutics is a privately held drug discovery company based in Basel, Switzerland. The company focuses on drugging key molecular targets involved in DNA Replication Stress, which constitutes an exciting and innovative approach in the development of novel anticancer drugs. FoRx is backed by a syndicate of investors that include the Novartis Venture Fund, Pfizer Ventures, M Ventures, Omega Funds and LSP Venture Capital. 

We are looking for a highly motivated and skilled Biochemist / Assay Development Scientist to join our growing team. This is an exciting opportunity to become an integral part of an innovative and dynamic start-up company. 

Key responsibilities of this role include, but are not limited to:

  • Expression and purification of recombinant proteins
  • Develop and perform a diverse set of biochemical assays, in particular enzymatic (e.g. enzyme activity and kinetics) and substrate binding assays
  • Design biophysics-grade protein constructs and pilot / contribute to the optimization of purification protocols
  • Screen small molecule ligands on purified protein in microplate-based assay formats to drive the understanding of SAR relationships using manual and automated systems
  • Crystallize or work in close collaboration with crystallographers to crystallize protein-ligand complexes in support of structure-aided drug development
  • Organize experimental results to be shared with project teams; maintain clear experimental records using ELN (electronic lab notebook)

Qualifications:

  • Recent PhD degree in Biochemistry or similar (e.g. Structural Biology)
  • 1-2 years of relevant experience in industry would be a strong plus
  • Profound experience and hands-on expertise in biochemical assays, enzyme kinetics and substrate binding assays
  • Experience in recombinant protein production using different expression systems, including construct design and optimization
  • Experience in using biophysical assays (SPR, ITC, DSF, etc.) for probing protein-ligand interactions
  • Expertise in X-ray protein crystallography would be an asset
  • First author publication in a relevant journal is required
  • Flexible, proactive, efficient, and able to work independently with a problem-solving attitude
  • Strong team player, high-energy and positive personality
  • Fluent in written and spoken English

The successful candidate will be an enthusiastic, self-driven individual who is flexible and responsive to the changing needs of a dynamic entrepreneurial organization, has the ability to communicate effectively, is an excellent fit to an agile and growing biotech team, and has the ambition to perform outstandingly.

FoRx Therapeutics encourages and supports a diverse work environment as an Equal Opportunity Employer, offering a stimulating work environment and a competitive compensation package.

Interested candidates should send a cover letter describing research interests and career goals, along with a Curriculum Vita to 

hr@forxtherapeutics.com

We thank all applicants for their interest, however only those candidates selected for interviews will be contacted. 


[Post Date: April 22, 2021]


Scientist I – Structural Biology ( X-ray crystallography)

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Description:

Job ID: req1845
Employee Type: exempt full-time
Facility: ATRF: Other
Location: 8560 Progress Drive, Frederick, MD 21701 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc.  The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Our core values of accountability, compassion, collaboration, dedication, integrity, and versatility serve as a guidepost for how we do our work every day in serving the public’s interest.  

 Position Overview:

PROGRAM DESCRIPTION

The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.

KEY ROLES/RESPONSIBILITIES

Scientist I – Structural Biology

  • The candidate will join the structural biology group within the RAS initiative and will be responsible for the crystallization and the structure determination of protein-small molecule and protein-protein complexes by X-ray crystallography
  • The work will include construct design, biochemical and biophysical characterization, crystallization, X-ray data collection, structure determination, and analysis
  • The candidate will work closely with protein scientists, biophysicists, and protein NMR scientists as part of an integrated structural biology effort
  • The candidate will be a member of the drug discovery team, working closely with computational and medicinal chemists on structure-based drug design approaches for small molecule agent optimization. The candidate will be expected to be able to participate in experimental design and to conduct experiments in a priority-driven environment independently
  • The candidate will be responsible for completing projects independently and as part of a team and will be responsible for timely updates of the project progress to their supervisor both informally and by formal report writing
  • Work requires the use of personal protective equipment and adherence to strict safety protocols

BASIC QUALIFICATIONS

  • Possession of a PhD degree in structural biology, protein biochemistry/biophysics, crystallography, or in a related discipline from an accredited college or university, according to the Council for Higher Education Accreditation
  • Proven ability in all aspects of protein crystallography from construct design through structure determination and analysis
  • Experience in the crystallization of protein-small molecule and protein-protein complexes
  • Expertise in commonly used structural biology software packages
  • Proficiency in protein biochemical and biophysical assays
  • Excellent communication and witting skills
  • Detail-oriented, highly organized and able to manage multiple tasks and deliver quality data
  • Must be able to obtain and maintain a clearance

PREFERRED QUALIFICATIONS

  • Experience in analyzing protein small-molecule interaction using isothermal titration calorimetry (ITC), differential scanning fluorimetry (DSF), and NMR techniques
  • Knowledge of mechanistic enzymology, and protein chemistry

JOB HAZARDS

  • This position is subject to working with radioactive material and requires to become an approved radiation worker

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)


[Post Date: April 21, 2021]


Team Leader (Structural Chemical Biology, Dundee—Almirall PROTAC collaboration)

View the Job Posting Here

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Description:

we are recruiting a Team Leader to lead a new team of five scientists as part of a drug discovery collaboration between my group at the School of Life Sciences at the University of Dundee, and Almirall (a global pharmaceutical company focused on skin diseases with high level of unmet medical need) and its Research Department located in Barcelona, Spain. The successful candidate and the team will be based in the new Dundee Centre for Targeted Protein Degradation.

https://www.dundee.ac.uk/stories/new-protein-degradation-centre-lead-drug-discovery-revolution 

We are looking for candidates with outstanding academic or industrial track record along with demonstrated ability to lead projects and teams. Relevant experience in structural biology and biophysical assays to study protein-ligand and protein-protein interactions.  Experience with PROTAC / targeted protein degradation drug discovery would be an advantage but is not essential. 

The post is available immediately and for a period of 3 years. Salary will depend on experience, but will be up to £49,553. This is a unique opportunity to gain experience in industry-style drug discovery within an academic setting.


[Post Date: April 20, 2021]


Structural Biology Postdoc Ohio State University

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Description:

Applications are invited for a joint postdoctoral position in the laboratories of Dr. Sarah Heissler and Dr. Krishna Chinthalapudi at the Ohio State University. The research project focuses on understanding the role of myosin motors and other cytoskeletal proteins in health and disease. We combine biochemical, biophysical and cell biological methods with high resolution imaging and state-of-the-art structural biology approaches including X-ray crystallography and cryo-EM to investigate how protein structure is related to its function.

Candidates must have a Ph.D. degree and strong practical experience in biochemistry and structural biology. Experiences with cell biology and high-resolution imaging are desirable.

To be considered for this position, please email your curriculum vitae, a short cover letter with research experience and interests and contact information for 3 references to Sarah Heissler (sarah.heissler@osumc.edu) and Krishna Chinthalapudi (krishna.chinthalapudi@osumc.edu).


[Post Date: April 20, 2021]