Image Credit: Gandhi, A.K., Sun, ZY.J., Kim, W.M. et al. Structural basis of the dynamic human CEACAM1 monomer-dimer equilibrium. Commun Biol 4, 360 (2021). DOI: 10.1038/s42003-021-01871-2.
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), is expressed on the surface of several cell types where it plays critical roles in morphogenesis, apoptosis, angiogenesis, cell proliferation cell motility, fibrosis, and most recently as an immunoreceptor important in mediating immune T cell tolerance. Crystal structures of hCEACAM1 IgV mutants were produced using crystals discovered using Hampton Research Index and PEGRx crystallization screens, including conditions containing Tacsimate, and optimized using the Additive Screen. The high-resolution structural studies and hCEACAM1 dynamic monomer–dimer equilibrium model provide a beneficial foundation towards therapeutic targeting of hCEACAM1 interactions with its various ligands.