Seeding allows one to grow crystals in the Metastable Zone, where spontaneous homogeneous nucleation cannot occur, but crystal growth from seeds can occur. Why would one want to do this? For control, reproducibility, and to improve the likelihood of a successful crystallization experiment. In the Metastable Zone crystals can grow from seeds but cannot spontaneously nucleate. By placing a seed or solution of seeds in a drop which is saturated to the Metastable Zone one can use the seeds to grow larger single crystals. By controlling the number of seeds introduced into the Metastable Zone drop one can control the number of crystals grown. It is not practically possible to measure and know the number of seeds introduced to a drop, but by performing serial dilutions from a concentrated seed stock one can control the number of crystals grown in the Metastable Drop.

The following crystallization presentations, posters, reading and products focus on seeding. Seeding can be a powerful screening and optimization method and should be in every crystal growers repertoire.

PDF of Nucleation and seeding in protein crystallization: an old story retold, by Allan D’Acry
Nucleation and seeding in protein crystallization: an old story retold, by Allan D’Acry

PDF of The importance of nucleation and seeding in protein crystallization, by Allan D'Arcy
The importance of nucleation and seeding in protein crystallization, case studies using microseed matrix seeding by Allan D'Arcy

PDF of Maximizing crystallization success with microseed matrix screening, by Galina Obmolova
Maximizing crystallization success with microseed matrix screening, by Galina Obmolova

PDF of A review of seeding methods: Old and New, by Aengus Mac Sweeney
A review of seeding methods, by Aengus Mac Sweeney

PDF of The benefits gained from a redesigned crystallization strategy focused on a high throughput seeding technique, by Alexey Rak
The benefits gained from a redesigned crystallisation strategy focused on a high throughput seeding technique, by Alexey Rak

PDF of Random microseeding: A theoretical and practical exploration of seed stability and seeding techniques for successful protein crystallization, by Patrick Shaw Stewart
Random microseeding: A theoretical and practical exploration of seed stability and seeding techniques for successful protein crystallization, by Patrick Shaw Stewart

PDF of Fast optimisation: 2-D grid seeding method, by Lesley F. Haire
Fast optimisation: 2-D grid seeding method, by Lesley F. Haire

PDF of Macro-seeding –a key to high resolution complex structures, by Hongwei Guo
Macro-seeding – a key to high resolution complex structures, by Hongwei Guo

PDF of Combining in-site proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes, by Romany Abskharon
Combining in-site proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes, by Romany Abskharon

PDF of Crystal Growth 101 – Seeding
Crystal Growth 101 – Seeding

Hampton Research Tools for Seeding

CAT NO NAME DESCRIPTION
HR2-320 Seed Bead Kit 24 tubes with PTFE Seed Bead
HR4-780 Seed Bead Steel Kit 24 tubes with Steel Seed Beads
HR4-781 Seed Bead Ceramic Kit 24 tubes with Ceramic Seed Beads
HR4-782 Seed Bead Glass Kit 24 tubes with Glass Seed Beads
HR4-216 Crystal Crusher 5 pack
HR4-217 Crystal Probe 12 pack
HR8-133 Seeding Tool 5 pack