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Search our Tips from A to Z for more information. Still having trouble finding what you want? Contact Hampton Research technical support.
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| V is for Vibration |
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| Vibration is often associated (in a crystallographers mind) with excessive nucleation. It is popular belief that the best crystals grow when there is NOT a significant source of vibration. It is believed this is particularly important during the optimization and production stages of crystallization. Hence, it is suggested that during optimization and production, one set crystallization experiments and avoid the burning curiosity to look at the plates every single day. Try leaving the setups alone during optimization and production.
Since vibration can lead to excessive nucleation, it seems one might want to move the plates some during screening. One typically does move plates during screening as it is typically recommended to view experiments each day for the first week and once a week thereafter until the drop dries out. Maybe we need to build vibrators into our screening incubators. Along the same line, it is sometimes observed that plates which have been sitting for some time, then viewed, often have crystals forming in the days following the latent observation. So perhaps one should consider giving the plates a Monty Python nudge-nudge or move them about to stimulate nucleation.
To avoid vibration, place plates in cupboards, cabinets, or incubators that are infrequently opened and closed. Installing hydraulic door dampers to prevent slamming doors or cupboards is one way to reduce vibration. Some incubators vibrate more than other units and peltier based heating and cooling incubators may be desirable from this perspective. However, some of the circulation fans in these units vibrate quite a bit, so be sure to check out the fan and even consider replacing it with a higher quality unit with less oscillation and vibration. |
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