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Search our Tips from A to Z for more information. Still having trouble finding what you want? Contact Hampton Research technical support.
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| O is for Organic Solvents |
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| The most popular volatile organic solvents used in biological macromolecular crystallization have been ethanol, acetone, isopropanol, tert-butanol, 1,3-propanediol, acetonitrile, DMSO, methanol, and 1,3-butyrolactone. Organic solvents can be utilized as a primary precipitant (buffered or unbuffered), as a secondary precipitant in the presence of salt or polymer (primary precipitant), or as additives. The most popular nonvolatile organics have been MPD and 1,6-hexanediol. Organic solvents act as precipitants by lowering the chemical activity of water. This means they steal water molecules from biological macromolecules in solution, through a process of hydrogen bonding. This in turn reduces the dieletric constant of the solution. Current popular thought is that organic solvents should be used at low temperatures (4°C or lower) and at the lowest possible ionic strength, keeping in mind to include whatever is necessary to stabilize the sample (buffer, divalent cations, etc.). |
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| O is for Osmium |
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| Osmium is a good reactant for ribose moieties and the 3’ terminus of RNA for heavy atom derivatives.
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TECHNICAL SUPPORT
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Tel: 949-425-1321
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Fax: 949-425-1611
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Email: tech@hrmail.com
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LOCATION
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Hampton Research
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Aliso Viejo, CA 92656-3317
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Toll Free: 800-452-3899
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Tel: 949-425-1321
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Fax: 949-425-1611
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