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Soak crystals overnight in a solution comprised of the mother liquor utilized to grow the crystals plus 0.5 to 2% v/v glutaraldehyde. Glutaraldehye cross-links proteins through the epsilon amino group of lysines. Cross-linking can be used to stabilize fragile crystals for seeding or data collection. Reference: F.A Quiocho and F.M. Richards. Proc. Natl. Acad. Sci. U.S.A. 52, 833 (1964).
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