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HEPES Buffer
Applications
Crystallization grade HEPES buffer for formulating screens or for optimization
Features
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HEPES
Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
C8H18N2O4S
Mr 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry Number 883043
Merck 14,4654
RTECS TL6809000
MDL Number MFCD00006158
PubChem Substance ID 24895616
Purity ≥99.5%
pKa 7.5 at 25°C

Useful pH range of HEPES buffer 6.8 – 8.2

HR2-585 can be titrated to the desired pH between 6.8 - 8.2 using NaOH (HR2-583)
HR2-585 Measured pH Range: 5.4 - 5.5 at 25°C
HR2-585 Measured Conductivity Range: 160.1 - 236.9 µS/cm at 25°C
HR2-585 Measured Refractive Index Range: 1.37093 - 1.37131 at 20°C

HR2-785 is titrated to pH 7.0 using NaOH
HR2-785 Measured Conductivity Range: 5.8 - 7.0 mS/cm at 25°C
HR2-785 Measured Refractive Index Range: 1.37133 - 1.37176 at 20°C

HR2-729 is titrated to pH 7.5 using NaOH
HR2-729 Measured Conductivity Range: 11.6 - 13.6 mS/cm at 25°C
HR2-729 Measured Refractive Index Range: 1.37177 - 1.37290 at 20°C

Insoluble Matter 0.01% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 1 ppm max
As 5 ppm max
Cu 5 ppm max
Fe 5 ppm max
Pb 5 ppm max
SO4 0.005% max
CAT NO NAME DESCRIPTION
HR2-585 HEPES - 1.0 M solution 100 ml
Price Quantity
$101.00
CAT NO NAME DESCRIPTION
HR2-785 HEPES pH 7.0 - 1.0 M solution 100 ml
Price Quantity
$101.00
CAT NO NAME DESCRIPTION
HR2-729 HEPES pH 7.5 - 1.0 M solution 100 ml
Price Quantity
$101.00
CAT NO NAME DESCRIPTION
HR2-583 1.0 M Sodium hydroxide 100 ml
Price Quantity
$32.90
References
1.Crystallization and preliminary X-ray crystallographic studies of native elastase from North Atlantic salmon (Salmo salar). Berglund, GI; Snalas, AO; Hansen, LK. Acta Crystallogr D 51, 393- 394, 1995.
Certificates of Analysis
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