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Imidazole Buffer
Imidazole Buffer
Applications
Crystallization grade Imidazole for formulating screens or for optimization
Features
Preformulated and sterile filtered
Lot to lot consistency
Consistent with Hampton Research screens & kits
Quality and convenience
Description
Optimize reagents are preformulated macromolecular crystallization grade reagents designed specifically for the crystallization of proteins, peptides, and nucleic acids. Each Optimize solution is formulated using high purity salts, polymers, and buffers. Sterile filtered Optimize reagents are formulated at convenient, ready to use concentrations. Optimize reagents remove the guesswork and make the process of reproducing preliminary screening conditions and general optimization faster, easier, and more convenient. When using Optimize reagents, a scientist moves directly from the screen to the optimization with no time wasted searching for and formulating salts, buffers, and viscous polymers. Each Optimize reagent is supplied with a Certificate of Analysis as documentation of reagent quality and performance. Optimize buffer reagents are supplied with calibrated titration curves to make titration easy, fast, and accurate.






Imidazole
Synonyms: 1,3-Diaza-2,4-cyclopentadiene and Glyoxaline.
C3H4N2
Mr 68.08
CAS Number [288-32-4]
EC Number 206-019-2
Beilstein Registry Number 103853
Merck 14,4912
RTECS NI3325000
MDL Number MFCD00005183
PubChem Substance ID 24880372
Purity > 99.5%
UN Number 2923 8/PG 3

pKa = 6.95
The useful pH range of an Imidazole can be 6.2-7.8

HR2-819 is titrated to pH 7.0 using HCl
HR2-819 Measured Conductivity Range: 45.4 - 50.4 mS/cm at 25°C
HR2-819 Measured Refractive Index Range: 1.34849 - 1.34904 at 20°C

Titrate HR2-573 to desired pH using HCl (Catalog number: HR2-581)
HR2-573 Measured pH Range: 10.5 - 10.7 at 25°C
HR2-573 Measured Conductivity Range: 72.9 - 101.5 µS/cm at 25°C
HR2-573 Measured Refractive Index Range: 1.34420 - 1.34438 at 20°C

Ca <0.001%
Cd <0.0005%
Cl <0.005%
Co <0.0005%
Cr <0.0005%
Cu <0.0005%
Fe <0.0005%
K <0.005%
Mg <0.0005%
Na <0.005%
Ni <0.0005%
Pb <0.0005%
SO4 <0.005%
Zn <0.0005%
ITEMS
CAT NO NAME DESCRIPTION
HR2-573 Imidazole - 1.0 M solution 100 ml
Price Quantity
$76.00
CAT NO NAME DESCRIPTION
HR2-819 1.0 M Imidazole pH 7.0 100 ml
Price Quantity
$90.00
1 Related Item
Hydrochloric acid
References
1.Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions. X. Qiu and C. A. Janson. Acta Cryst. (2004). D60, 1545-1554 [ doi:10.1107/S0907444904015422 ]
2.Protein crystallization by surface entropy reduction: optimization of the SER strategy. D. R. Cooper, T. Boczek, K. Grelewska, M. Pinkowska, M. Sikorska, M. Zawadzki and Z. Derewenda. Acta Cryst. (2007). D63, 636-645 [ doi:10.1107/S0907444907010931 ]
Certificates of Analysis
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