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HEPES sodium Buffer
HEPES sodium Buffer
Applications
Crystallization grade HEPES sodium buffer for formulating screens or for optimization
Features
Preformulated and sterile filtered
Lot to lot consistency
Consistent with Hampton Research screens & kits
Quality and convenience
Description
Optimize reagents are preformulated macromolecular crystallization grade reagents designed specifically for the crystallization of proteins, peptides, and nucleic acids. Each Optimize solution is formulated using high purity salts, polymers, and buffers. Sterile filtered Optimize reagents are formulated at convenient, ready to use concentrations. Optimize reagents remove the guesswork and make the process of reproducing preliminary screening conditions and general optimization faster, easier, and more convenient. When using Optimize reagents, a scientist moves directly from the screen to the optimization with no time wasted searching for and formulating salts, buffers, and viscous polymers. Each Optimize reagent is supplied with a Certificate of Analysis as documentation of reagent quality and performance. Optimize buffer reagents are supplied with calibrated titration curves to make titration easy, fast, and accurate.

HEPES sodium
Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt
C8H17N2O4NaS
Mr 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6-8.2

HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 42.6 - 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37648 - 1.37766 at 20°C

HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 11.4 - 12.1 at 25°C
HR2-577 measured Conductivity range: 27.0 - 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37249 - 1.37298 at 20°C

HR2-931-01 - 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.
ITEMS
CAT NO NAME DESCRIPTION
HR2-577 1.0 M HEPES sodium 100 ml solution
Price Quantity
$109.00
CAT NO NAME DESCRIPTION
HR2-733 1.0 M HEPES sodium pH 7.5 100 ml solution
Price Quantity
$109.00
CAT NO NAME DESCRIPTION
HR2-931-01 1.0 M HEPES sodium pH 6.8 185ml
Price Quantity
$204.00
References
1.Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.
Certificates of Analysis
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