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Proti-Ace & Proti-Ace 2 Individual Reagents
Proti-Ace & Proti-Ace 2 Individual Reagents


Applications
In situ proteolysis and proteolytic screening of protein samples for crystallization and structure determination
Individual reagents from Proti-Ace & Proti-Ace 2 kits
Features
Lyophilized enzymes for enhanced stability
Description
Individual reagents from the Proti-Ace and Proti-Ace 2 kits.
Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

The unique freeze dried formulation of the Proti-Ace kit offers a much improved protease stability compared to liquid protease formulations. Each tube contains 0.1 mg of lyophilized enzyme and digest buffer as shown below. Add 100 microliters of deionized water (Type 1+) to each tube of lyophilized content to create a 1 mg/ml protease solution in digest buffer. Proti-Ace Dilution buffer is supplied as a ready to use solution.

Proti-Ace Reagent 1* (Qty 3):
• 1 mg/ml a-Chymotrypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 2* (Qty 3):
• 1 mg/ml Trypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 3* (Qty 3):
• 1 mg/ml Elastase, 200 mM Tris pH 8.0
Proti-Ace Reagent 4* (Qty 3):
• 1 mg/ml Papain, 2 mM EDTA, 2 mM TCEP hydrochloride, 2 mM L-Cysteine
Proti-Ace Reagent 5* (Qty 3):
• 1 mg/ml Subtilisin, 10 mM Sodium acetate trihydrate pH 7.5, 5 mM Calcium acetate hydrate
Proti-Ace Reagent 6* (Qty 3):
• 1 mg/ml Endoproteinase Glu-C (in deionized water)
* When 100 µl of deionized water is added to each Proti-Ace freeze dried reagent

Proti-Ace Dilution Buffer (Qty 3):
• 1.6 ml of 10 mM HEPES pH 7.5, 500 mM Sodium chloride

Proti-Ace 2 Reagent 1* (Qty 3):
• 1 mg/ml Proteinase K, 10 mM TRIS hydrochloride pH 7.5, 1 mM Calcium acetate hydrate
Proti-Ace 2 Reagent 2* (Qty 3):
• 1 mg/ml Clostripain (Endoproteinase-Arg-C), 10 mM HEPES pH 7.5, 2 mM TCEP hydrochloride, 2 mM L-Cysteine,
1 mM Calcium chloride dihydrate
Proti-Ace 2 Reagent 3* (Qty 3):
• 1 mg/ml Pepsin, 1 mM Hydrochloric acid
Proti-Ace 2 Reagent 4* (Qty 3):
• 1 mg/ml Thermolysin, 10 mM Calcium acetate hydrate pH 8.0, 50 mM Sodium chloride
Proti-Ace 2 Reagent 5* (Qty 3):
• 1 mg/ml Bromelain, 5 mM HEPES pH 7.5, 2 mM L-Cysteine, 1 mM Ethylenediaminetetraacetic acid disodium salt dihydrate
Proti-Ace 2 Reagent 6* (Qty 3):
• 1 mg/ml Actinase, 5 mM HEPES pH 7.5, 10 mM Sodium chloride
* When 100 µl of deionized water is added to each Proti-Ace 2 freeze dried
reagent
CAT NO NAME DESCRIPTION
HR2-429-01 Proti-Ace™ Reagent 1 a-Chymotrypsin - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-429-02 Proti-Ace™ Reagent 2 Trypsin - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-429-03 Proti-Ace™ Reagent 3 Elastase - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-429-04 Proti-Ace™ Reagent 4 Papain - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-429-05 Proti-Ace™ Reagent 5 Subtilisin - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-429-06 Proti-Ace™ Reagent 6 Endoproteinase Glu-C - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-429-07 Proti-Ace™ Dilution Buffer pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-432-01 Proti-Ace™ 2 Reagent 1 Proteinase K - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-432-02 Proti-Ace™ 2 Reagent 2 Clostripain (Endoproteinase-Arg-C) - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-432-03 Proti-Ace™ 2 Reagent 3 Pepsin - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-432-04 Proti-Ace™ 2 Reagent 4 Thermolysin - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-432-05 Proti-Ace™ 2 Reagent 5 Bromelain - pack of 3 tubes
Price Quantity
$64.00
CAT NO NAME DESCRIPTION
HR2-432-06 Proti-Ace™ 2 Reagent 6 Actinase E - pack of 3 tubes
Price Quantity
$64.00
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