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Home  |   Products | Crystallization Plates, Hardware & Accessories | 96 Well Crystallization Plates | Vapor Batch 96 Well Plate (Douglas Instruments)
Vapor Batch 96 Well Plate (Douglas Instruments)
Vapor Batch 96 Well Plate (Douglas Instruments)
Vapor Batch 96 Well Plate (Douglas Instruments)


Applications
Sitting drop crystallization, microbatch crystallization
Anaerobic crystallization
Crystallization using volatile organics
Features
96 conical, flat bottom wells
Compact size
Supplied with cover
Microbatch and vapor diffusion from the same plate
Hydrophobic or hydrophilic versions
Controlled drop dehydration
Materials
Polystyrene
Description
The Douglas Instruments Vapor Batch Plate is designed for both microbatch and sitting drop vapor diffusion crystallization. It has 96 wells in the central region of the plate and several reservoirs around the outside of the plate. Oil is placed in the central region followed by sample and reagent for a microbatch experiment. When the outside reservoirs are filled with reagent, they can be used to preserve microbatch crystals by preventing the drops from drying out. The reservoirs can also be used for common dehydrant, sitting drop vapor diffusion experiments where up to 96 wells are equilibrated against a single reagent or dehydrant. Preliminary experiments suggest this method may find more hits in screening experiments than the conventional method of using the same solution in the reservoir and the drop. Wells are individually labeled. The Vapor Batch Plate is available as a plasma-treated hydrophilic version for screening or an untreated, hydrophobic version for optimization. The hydrophilic version gives better liquid handling with small volumes, while the hydrophobic version reduces crystal nucleation and helps to prevent the crystals from sticking to the plastic. Vapor Batch Plate Holders which provide a Linbro®, VDX™, or SBS footprint are available on a special order basis. Approximate plate dimensions: 81 mm x 55 mm x 20 mm. Drop well depth: 1.8 mm. Typical drop volume: 1 to 9 µl. Maximum drop volume: 20 µl. Recommended oil fill: 2.5 ml. Recommended reagent/dehydrant volume: 8 ml.

The humidity within the Vapor Batch plate can be controlled by the vapor pressure of the liquid in the reservoirs. Most Rapid Dehydration = Dry < 2 M NaCl < 0.5 M NaCl < DI water = Slowest Dehydration. The rate of dehydration is dependent upon the reservoir, the oil, drop volume and crystallization reagent.

ITEMS
CAT NO NAME DESCRIPTION
DI-038 Vapor Batch Plate, treated, hydrophilic 10 plate case
Price Quantity
$68.00
CAT NO NAME DESCRIPTION
DI-040 Vapor Batch Plate, treated, hydrophilic 80 plate case
Price Quantity
$485.00
CAT NO NAME DESCRIPTION
DI-039 Vapor Batch Plate, untreated, hydrophobic 10 plate case
Price Quantity
$68.00
CAT NO NAME DESCRIPTION
DI-041 Vapor Batch Plate, untreated, hydrophobic 80 plate case
Price Quantity
$485.00
References
1.Control of the rate of evaporation in protein crystallization by the `microbatch under oil' method. B. Brumshtein, H. M. Greenblatt, A. H. Futerman, I. Silman and J. L. Sussman. J. Appl. Cryst. (2008). 41, 969-971.
2.High-resolution structure of a retroviral capsid hexameric amino-terminal domain. Mortuza, Nature 431 (2004), pp 481-485.
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