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Detergent Screen • Detergent Screen HT
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Detergent Screen • Detergent Screen HT
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| Crystal of beta-lactamase grown in the presence of detergent. James Knox, University of Connecticut |
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| Detergent molecule interacting with beta-lactamase. |
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| A tetragonal crystal of AppA, a photoreceptor from Rhodobacter sphaeroides, grown in Hampton Research Detergent Screen. Vladimira Dragnea, Indiana University. |
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Prevent and manipulate non-specific aggregation due to hydrophobic interactions
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Kits include popular detergents used in the crystallization of membrane proteins
 | | | Ionic detergents |
| | | Non-ionic detergents |
| | | Zwitterionic detergents |
| | | Non-detergent Sulfobetaines |
| | | Synthetic lipids |
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Useful with soluble proteins where hydrophobic interactions limit sample solubility
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Detergents are compatible with microbatch under oil crystallization, as well as vapor diffusion, free interface diffusion and dialysis
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Tube or Deep Well block format
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Detergent Screen kits are designed to allow the rapid and convenient evaluation of a total of 96 unique detergent reagents for their ability to influence the solubility and crystallization of the sample. These screens are designed to be compatible with most popular crystallization reagents.
The detergents in these kits are capable of manipulating hydrophobic sample-sample interactions which can lead to non-specific aggregation, and prevent or interfere with sample crystallization. The detergents also perturb water structure which may play a role in sample crystallization.
Non-specific aggregation is a common deterrent to the crystallization of soluble macromolecules as well as membrane proteins. There is extensive literature demonstrating the effectiveness of including detergents in the crystallization trial towards preventing non-specific aggregation due to hydrophobic interactions and hence improving crystallization.1-7 The kits allow one to screen the most effective detergents used in crystal growth.
The Detergent Screen kits are preformulated so that simple pipetting is all that is required to screen the detergents with the hanging or sitting drop vapor diffusion technique. Screens with these kits are usually performed after preliminary crystallization conditions have been determined, although ab initio screens are also practical. The kits are recommended for both soluble and membrane proteins where non-specific aggregation is a suspected complication or where one simply wishes to screen detergents as an optimization variable.
The screens are suitable for hanging drop, sitting drop, microbatch, free interface diffusion and sandwich drop crystallization methodologies. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity detergents and are filled under argon gas to enhance stability.
Each Detergent Screen 1, 2 or 3 kit contains 24 unique detergents. 100 µl of detergent solution is preformulated at 10 times the reported CMC (unless otherwise noted) in sterile filtered water.
Each Detergent Screen kit contains 96 unique detergents. 0.25 ml of detergent solution is preformulated at 10 times the reported CMC (unless otherwise noted) in sterile filtered water. The Detergent Screen kit contains 96 solutions in o-ring screw cap tubes.
Each Detergent Screen HT kit contains 96 unique detergents. 0.25 ml of detergent solution is preformulated at 10 times the reported CMC (unless otherwise noted) in sterile filtered water. The Detergent Screen HT kit contains 96 solutions in a single Deep Well block.
For Lot Number 241113 Detergent Screen 2 reagent 24 was changed from C-HEGA-8 to CYPFOS-3. All other lots of Detergent Screen 2 used C-HEGA-8 for reagent number 24.
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-410 |
Detergent Screen 1 |
24 tubes, 0.1 ml each |
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-411 |
Detergent Screen 2 |
24 tubes, 0.1 ml each |
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-412 |
Detergent Screen 3 |
24 tubes, 0.1 ml each |
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-408 |
Detergent Screen |
0.25 ml, tube format |
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-406 |
Detergent Screen HT |
0.25 ml, Deep Well block format |
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References
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| 1. | Crystallization of membrane proteins. Edited by Hartmut Michel, CRC Press, (1991). |
| 2. | Crystallization of Nucleic Acids and Proteins: A Practical Approach. Oxford University Press, Pages 175-191 (1992). |
| 3. | McPherson, A., et al., The effects of neutral detergents on the crystallization of soluble proteins., J. Crystal Growth (1986) 76, 547-553. |
| 4. | Kuhlbrand, W., Quarterly Rev. Biophys. (1988) 21, 429. |
| 5. | Garavito, R.M., & Picot, D., Methods, A Companion to Methods in Enzymology (1990) 1, 57. |
| 6. | Garavito, R.M,. et al., J. Crystal Growth (1986) 76, 701-709. 7. Cudney, R., et al., Acta Cryst. (1994) D50, 414-423. |
| 7. | Thomas R.M. Barenda and Bauke W. Diskstra. Oils used in microbatch crystallization do not remove a detergent from the drops they cover. Acta Cryst. (2003). D59, 2345-2347. |
| 8. | Loll et al. Compatibility of detergents with the microbatch-under-oil crystallization method. Acta Cryst. (2003). D59, 114-116. |
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