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Nucleic Acid Mini Screen (NAM)
Nucleic Acid Mini Screen (NAM)


Applications
Crystallization screen for nucleic acid fragments
Features
Screen a matrix of pH, polyamine, & ions
Can be used with ribozymes, pseudoknots, RNA, & DNA
Uses MPD as the primary precipitant
Description
The Nucleic Acid Mini Screen is an efficient screen formulated to assist in the determination of preliminary crystallization conditions of nucleic acid fragments.1 The formulation is based upon the publication, “A Highly Efficient 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments” where the preliminary crystallization conditions of 35 nucleic acids were determined utilizing this formulation.1 Samples include DNA-Drug complexes, C-Tetrad and G-Quartet Motifs, RNA oligomers, and others. By using 1 to 4 mM oligonucleotide stock concentration, the screen requires less than 100 µl of sample. The screen is typically performed at 4°C although room temperature incubations can also be performed. To evaluate the effect of equilibration kinetics as well as initial and final sample concentrations, the screens are typically performed using the hanging or sitting drop vapor diffusion method with two drops on a slide (1 µl + 2 µl and 2 µl + 2 µl), side by side. The 24 well format of the mini screen provides for a fast setup and uses small amounts of sample. This makes it possible to cost-effectively screen many sequences as well as variations of a particular sequence. The composition of the Nucleic Acid Mini Screen allows one to apply the formulation to other nucleic acids such as deoxy- and ribozymes, pseudoknots, and tRNAs.

Each Nucleic Acid Mini Screen kit contains 24 unique reagents, 1.0 ml each plus a 250 ml volume of dehydrant (35% v/v MPD). All solutions are formulated using ultra-pure Type 1 water and are sterile filtered.
CAT NO NAME DESCRIPTION
HR2-118 Nucleic Acid Mini Screen 1 ml, tube format + 250 ml bottle
Price Quantity
$251.00
References
1.Berger, et al., A Highly Effective 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments. Acta Cryst. Section D. (1996) Vol. D52 Part 3, 465-468.
2.Adams, A., Nucleic Acids Research (2002) Vol. 30, 719-725.
3.Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Förster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Fürste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.
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