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Proti-Ace • Proti-Ace 2
Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2
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Proti-Ace • Proti-Ace 2
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Applications
In situ proteolysis and proteolytic screening of protein samples for crystallization and structure determination
Features
Proti-Ace proteases
a-Chymotrypsin
Trypsin
Elastase
Papain
Subtilisin
Endoproteinase Glu-C
Proti-Ace 2 proteases
Proteinase K
Clostripain (Endoproteinase-Arg-C)
Pepsin
Thermolysin
Bromelain
Actinase E
Optimized, stable, freeze dried protease formulation
Enhanced stability
Proti-Ace Dilution Buffer
Optimized protocol for in situ proteolysis or proteolytic screening
Description
A proteolytic fragment or domain of a protein may crystallize more readily or form better diffracting crystals than the intact protein.1-8 Proteases can be used to generate small, active fragments or domains of the target protein for crystallization.9 The fragment or domain can be used directly for crystallization experiments. Or the proteolytic sample analyzed by gel electrophoresis and/or mass spectrometry for mass and sequence for subsequent cloning, expression, purification and crystallization. Using proteolysis to enhance sample crystallization, the current overall success rate for yielding a deposited crystal structure is currently better than 12%.3

Proti-Ace (HR2-429) contains three aliquots of 6 unique proteases (a-Chymotrypsin, Trypsin, Elastase, Papain, Subtilisin and Endoproteinase Glu-C) and six aliquots of Proti-Ace Dilution Buffer. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

Proti-Ace 2 (HR2-432) contains three aliquots of 6 unique proteases (Proteinase K, Clostripain (Endoproteinase-Arg-C), Pepsin, Thermolysin, Bromelain and Actinase E) and six aliquots of Proti-Ace Dilution Buffer. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

The unique freeze dried formulation of the Proti-Ace kits offers a much improved protease stability compared to liquid protease formulations.
CAT NO NAME DESCRIPTION
HR2-429 Proti-Ace™ Kit
Price Quantity
$221.00
CAT NO NAME DESCRIPTION
HR2-432 Proti-Ace™ 2 Kit
Price Quantity
$221.00
References
1.Allan D’Arcy, personal communication, 1989-2009
2.In situ proteolysis for protein crystallization and structure determination. Dong, A et al. Nature Methods - 4, 1019 - 1021 (2007)
3.In Situ Proteolysis to Generate Crystals for Structure Determination: An Update. Amy Wernimont, Aled Edwards. PLoS ONE 4(4): e5094. doi:10.1371/ journal.pone.0005094
4.The use of in situ proteolysis in the crystallization of murine CstF-77. Tong et al. Acta Cryst. (2007). F63, 135-138
5.A brief history of protein crystal growth. McPherson, A. Journal of Crystal Growth, vol. 110, issue 1-2, pp. 1-10, 1991
6.Preparation and analysis of protein crystals. McPherson, A. John Wiley & Sons, Inc. 1982. ISBN 089464355X
7.A crystallizable form of the Streptococcus gordonii surface antigen SspB C-domain obtained by limited proteolysis. Forsgren et al. Acta Cryst. (2009). F65, 712–714
8.Preliminary X-ray analysis of a human VH fragment at 1.8 angstrom resolution. Gaur, Kupper, Fischer & Hoffman. Acta Cryst. (2004). D60, 965-967
9.Replication Protein A Characterization and Crystallization of the DNA Binding Domain. Pfuetzner et al. The Journal of Biological Chemistry, Vol. 272, No. 1, Issue of January 3, pp. 430–434, 1997.
10.Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB. Little et al. Acta Cryst. (2012) F68, 842-845.
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