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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
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| Crystals of Peptidoglycan Recognition Protein (PGRP) from camel milk grown using the Hampton Research PEG/Ion screen. Sharma, P., Singh, N., Sinha, M., Sharma, S., Perbandt, M., Betzel, C., Kaur, P., Srinivasan, A. & Singh, T.P. All India Institute of Medical Sciences, Department of Biophysics, New Delhi, India. |
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Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
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PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 - 9.2
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PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 - 8.8) in the presence of monodisperse PEG 3,350
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PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block
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PEG/Ion, PEG/Ion 2 and PEG/Ion HT were developed at Hampton Research
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PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.
PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 - 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.
PEG/Ion contains 48 unique reagents, 10 ml each.
PEG/Ion 2 contains 48 unique reagents, 10 ml each.
PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
PEG/Ion 2
Measured pH range of kit is 3.7 to 8.8 at 25°C
Average measured pH of kit is 6.4 at 25°C
Median measured pH of kit is 6.7 at 25°C
Mode measured pH of kit is 6.7 at 25°C
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-126 |
PEG/Ion Screen |
10 ml, tube format |
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-098 |
PEG/Ion 2 Screen |
10 ml, tube format |
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CAT NO |
NAME |
DESCRIPTION |
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| HR2-139 |
PEG/Ion HT |
1 ml, Deep Well block format |
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References
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| 1. | Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase. C. V. dos Reis, A. Bernardes and I. Polikarpov. Acta Cryst. (2013). F69, 588-591. |
| 2. | A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422. |
| 3. | Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology Volume 156, Issue 3 , December 2006, Pages 387-406 |
| 4. | Mechanisms for Glycolipid Antigen-Driven Cytokine Polarization by V{alpha}14i NKT Cells. Sulliivan et al, The Journal of Immunology, 2010, 184, 141 -153. (PEG/Ion 2) |
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